| Project/Area Number |
26460053
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Shujitsu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SAITO Keita 就実大学, 薬学部, 講師 (30454854)
ISHIZAKI Atsusi 就実大学, 薬学部, 助教 (20780993)
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| Research Collaborator |
MIZUNO Keisuke 就実大学, 薬学部, 学生
MORIYAMA Eri 就実大学, 薬学部, 学生
UEMURA Akiko 就実大学, 薬学部, 学生
SAITO Akihiro 就実大学, 薬学部, 学生
HARA Yukari 就実大学, 薬学部, 学生
HAMANO Mariko 就実大学, 薬学部, 学生
MIURA Akiko 就実大学, 薬学部, 学生
KITAGAWA Sae 就実大学, 薬学部, 学生
MUNEOKA Tetsuya 就実大学, 薬学部, 学生
MAITOKO Hiroto 就実大学, 薬学部, 学生
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 固相マイクロ抽出法 / オンライン自動分析 / 生体酸化ストレス / バイオマーカー / LC-MS/MS分析 / 糖化ストレス / タバコ煙曝露 / 非侵襲的分析 / 毛髪分析 / 8-OHdG / 8-イソプロスタン / 3-ニトロチロシン / 酸化ストレス / LC－MS/MS分析 |
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| Outline of Final Research Achievements |
We developed an automated on-line analytical method for the simultaneous determination of multiple biomarkers for oxidative stress by in-tube SPME coupled with negative/positive switching ion-mode liquid chromatography-tandem mass spectrometry (LC-MS/MS). In-tube SPME and LC-MS/MS conditions were optimized for 8-isoprostane, 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 3-nitro-L-tyrosine, which are biomarkers of endogenous oxidative damage to lipids, DNA and proteins, respectively. The in-tube SPME/LC-MS/MS method is simple, rapid, selective and sensitive, and was applied successfully to the noninvasive analyses of trace amount of oxidative stress and related biomarkers in urine, saliva and hair samples without any other pretreatment and without interference peaks. This method is useful tool as a comprehensive evaluation method of health effects due to smoking and other lifestyle, and it is expected to be applied to prevention and early diagnosis of oxidative stress related diseases.
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