| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Diabetic patients are sensitive to infection caused by Staphylococcus aureus and the infectious diseases become severe. However, the molecular mechanisms in S. aureus to adapt the host environment to cause the severe infection in diabetic patients were unknown. In this study, we performed comprehensive gene expression analysis of S. aureus in the body of diabetic silkworm and normal silkworm, and identified genes involved in metabolic pathways necessary for adapting to the diabetic host environment. Comprehensive gene expression analysis showed that expression of the genes encoding enzymes involved in the synthesis of branched chain amino acids of S. aureus was elevated in diabetic silkworm. These gene-disrupted mutants are necessary for infection against diabetic silkworms but are not required for that against normal silkworms. These results suggest that Staphylococcus aureus adapts to diabetic host conditions via elevated branched chain amino acid synthesis.
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