Comprehensive understanding of bacterial adaptation mechanism in diabetic host
Project/Area Number |
26460062
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biological pharmacy
|
Research Institution | Teikyo University (2016) The University of Tokyo (2014-2015) |
Principal Investigator |
|
Co-Investigator(Renkei-kenkyūsha) |
SEKIMIZU KAZUHISA 帝京大学, 医真菌研究センター, 教授 (90126095)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 黄色ブドウ球菌 / 感染症 / カイコ / 糖尿病 / 高血糖 / トランスクリプトーム解析 |
Outline of Final Research Achievements |
Diabetic patients are sensitive to infection caused by Staphylococcus aureus and the infectious diseases become severe. However, the molecular mechanisms in S. aureus to adapt the host environment to cause the severe infection in diabetic patients were unknown. In this study, we performed comprehensive gene expression analysis of S. aureus in the body of diabetic silkworm and normal silkworm, and identified genes involved in metabolic pathways necessary for adapting to the diabetic host environment. Comprehensive gene expression analysis showed that expression of the genes encoding enzymes involved in the synthesis of branched chain amino acids of S. aureus was elevated in diabetic silkworm. These gene-disrupted mutants are necessary for infection against diabetic silkworms but are not required for that against normal silkworms. These results suggest that Staphylococcus aureus adapts to diabetic host conditions via elevated branched chain amino acid synthesis.
|
Report
(4 results)
Research Products
(57 results)
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[Journal Article] Synergistic effects of vancomycin and beta-lactams against vancomycin highly resistant Staphylococcus aureus.2017
Author(s)
Tabuchi, F., Matsumoto, Y., Ishii, M., Tatsuno, K., Okazaki, M., Sato, T., Moriya, K., and Sekimizu, K.
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Journal Title
J Antibiot (Tokyo)
Volume: 印刷中
Issue: 6
Pages: 771-774
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Phenotypic and genomic comparisons of highly vancomycin-resistant Staphylococcus aureus strains developed from multiple clinical MRSA strains by in vitro mutagenesis.2015
Author(s)
Ishii K, Tabuchi F, Matsuo M, Tatsuno K, Sato T, Okazaki M, Hamamoto H, Matsumoto Y, Kaito C, Aoyagi T, Hiramatsu K, Kaku M, Moriya K, Sekimizu K.
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Journal Title
Sci Rep
Volume: 5
Issue: 1
Pages: 17092-17092
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Transgenic silkworms expressing human insulin receptors for evaluation of therapeutically active insulin receptor agonists.2014
Author(s)
Matsumoto Y, Ishii M, Ishii K, Miyaguchi W, Horie R, Inagaki Y, Hamamoto H, Tatematsu K, Uchino K, Tamura T, Sezutsu H, Sekimizu K.
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 455(3-4)
Issue: 3-4
Pages: 159-164
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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