| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We analyzed mechanism of substrate transport by ABCD1-3 on peroxisomal membrane and mechanism of translocation of ABCD4 to lysosome.
It is revealed that ABCD1 possesses acyl-CoA thioesterase activity and its activity is essential for the transport of acyl-CoA into peroxisome. In addition, it is revealed that ABCD4 makes complex with lysosomal protein LMBD1 on endoplasmic reticulum membranes and translocates from ER to lysosome dependent on lysosomal targeting signal of LMBD1. Furthermore, we succeeded to prepare proteoliposome of ABCD1-4. It becomes possible to characterized precise mechanism of substrate transport by ABCD1-4.
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