The functional analyses of fad104, a novel regulator of osteoblast differentiation.

• Project/Area Number: 26460074
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: Nishizuka Makoto 名古屋市立大学, 大学院薬学研究科, 講師 (00363953)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: FAD104 / 骨分化 / 脂肪細胞分化 / がん細胞 / Smad / STAT3 / 骨形成

Outline of Final Research Achievements

In this study, we demonstrated that the amino acid region 161-277 of FAD104 is required for interaction with Smad1, a regulator of osteoblast differentiation. Furthermore, knockdown and overexpression experiments showed that FAD104 suppressed the invasion, metastasis and anchorage-independent growth of cancer cells. In addition, we demonstrated that the N-terminal region of FAD104 is essential for inhibiting STAT3 activity.

Research Products

• [Journal Article] KCNMA1, a pore-forming α-subunit of BK channels, regulates insulin signalling in mature adipocytes (2016)
  • Author(s): 41)Nishizuka M, Horinouchi W, Yamada E, Ochiai N, Osada S, Imagawa M.
  • Journal Title: FEBS Lett. Volume: 590 Pages: 4372-4372
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] FAD104, a regulator of adipogenesis and osteogenesis, interacts with the C-terminal region of STAT3 and represses malignant transformation of melanoma cells. (2016)
  • Author(s): Katoh D, Nishizuka M, Osada S, Imagawa M.
  • Journal Title: Biol Pharm Bull Volume: 印刷中
  • NAID: 130005149249
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Fad24, a positive regulator of adipogenesis, is required for S phase re-entry of C2C12 myoblasts arrested in G0 phase and involved in p27Kip1 expression at the protein level. (2016)
  • Author(s): Ochiai N, Nishizuka M, Osada S, Imagawa M.
  • Journal Title: Biol Pharm Bull Volume: 印刷中
  • NAID: 130005149257
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Fad104, a positive regulator of adipocyte differentiation, suppresses invasion and metastasis of melanoma cells by inhibition of STAT3 activity. (2015)
  • Author(s): Katoh D, Nishizuka M, Osada S, Imagawa M.
  • Journal Title: Plos One Volume: 10 Issue: 2 Pages: e0117197-e0117197
  • DOI: 10.1371/journal.pone.0117197
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] KCNK10, a tandem pore domain potassium channel, is a regulator of mitotic clonal expansion during the early stage of adipocyte differentiation. (2014)
  • Author(s): Nishizuka M, Hayashi T, Asano M, Osada S, Imagawa M.
  • Journal Title: Int. J. Mol. Sci. Volume: 15 Issue: 12 Pages: 22743-22756
  • DOI: 10.3390/ijms151222743
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] TGF-b誘導性EMTにおける脂肪細胞分化制御因子fad104の機能解析 (2017)
  • Author(s): 後藤元晴、長田茂宏、西塚 誠、今川正良
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-27
• [Presentation] The role of fad104, a positive regulator for adipogenesis, in TGF-b1-induced epithelial to mesenchymal transition. (2016)
  • Author(s): Goto M., Nishizuka M., Osada S., Imagawa M.
  • Organizer: 2016 American Society Cell Biology Annual Meeting
  • Place of Presentation: San Francisco
  • Year and Date: 2016-12-06
  • Int'l Joint Research
• [Presentation] KCNMA1 contributes to the regulation of insulin signaling in mature adipocytes. (2016)
  • Author(s): Nishizuka M., Horinouchi W., Yamada E., Osada S., Imagawa M.
  • Organizer: 2016 American Society Cell Biology Annual Meeting
  • Place of Presentation: San Francisco
  • Year and Date: 2016-12-05
  • Int'l Joint Research
• [Presentation] 脂肪細胞におけるカリウムチャネルの役割 (2016)
  • Author(s): 西塚 誠、中野友香、成田沙智世、長田茂宏、今川正良
  • Organizer: 第38回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2016-11-18
• [Presentation] がん細胞の上皮間葉転換における脂肪細胞分化促進因子fad104の役割 (2016)
  • Author(s): 後藤元晴、長田茂宏、西塚 誠、今川正良
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2016
  • Place of Presentation: 岐阜
  • Year and Date: 2016-10-30
• [Presentation] 脂肪細胞分化過程における各種カリウムチャネルの発現と役割の検討 (2016)
  • Author(s): 中野友香、成田沙智世、長田茂宏、西塚 誠、今川正良
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2016
  • Place of Presentation: 岐阜
  • Year and Date: 2016-10-30
• [Presentation] 低分子量Gタンパク質RhoEの上皮間葉転換ならびに脂肪細胞分化過程における発現検討 (2016)
  • Author(s): 駒田莉奈、西塚 誠、長田茂宏、今川正良
  • Organizer: 第62回日本薬学会東海支部総会・大会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-07-09
• [Presentation] 白色脂肪組織ならびに脂肪細胞分化過程における各種カリウムチャネルの発現 (2016)
  • Author(s): 成田沙智世、中野友香、長田茂宏、西塚 誠、今川正良
  • Organizer: 第62回日本薬学会東海支部総会・大会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-07-09
• [Presentation] 脂肪細胞分化関連遺伝子fad104は上皮間葉転換を制御する (2016)
  • Author(s): 後藤元晴、加藤大輝、長田茂宏、西塚 誠、今川正良
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-28
• [Presentation] 脂肪細胞分化関連因子fad104によるSTAT3シグナルの制御機構の解析 (2015)
  • Author(s): 加藤大輝, 西塚誠, 長田茂宏, 今川正良
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-03
• [Presentation] 脂肪細胞分化促進因子fad24は筋芽細胞の増殖再開を正に制御する (2015)
  • Author(s): 落合なつき, 西塚誠, 長田茂宏, 今川正良
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-03
• [Presentation] Fad104, a positive regulator of adipogenesis, inhibits invasion and metastasis of cancer cells through the suppression of STAT3 activity (2015)
  • Author(s): Katoh D., Nishizuka M., Osada S., Imagawa M.
  • Organizer: 40th FEBS Congress
  • Place of Presentation: Berlin
  • Year and Date: 2015-07-08
• [Presentation] The role of KCNMA1 in mature adipocytes (2015)
  • Author(s): Nishizuka M., Horinouchi W., Yamada E., Osada S., Imagawa M.
  • Organizer: 40th FEBS Congress
  • Place of Presentation: Berlin
  • Year and Date: 2015-07-08
• [Presentation] The role of fad24, a positive regulator for adipogenesis, in early embryonic development and muscle cell activation (2015)
  • Author(s): Ochiai N., Nishizuka M., Osada S., Imagawa M.
  • Organizer: 40th FEBS Congress
  • Place of Presentation: Berlin
  • Year and Date: 2015-07-08
• [Presentation] TGF-betaによる上皮間葉転換にfad104が与える影響 (2015)
  • Author(s): 後藤元晴、加藤大輝、長田茂宏、西塚 誠、今川正良
  • Organizer: 第61回日本薬学会東海支部総会・大会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-07-04
• [Presentation] 新規脂肪細胞分化関連因子FAD24がNF-kBを介するプロモーター活性に与える影響 (2015)
  • Author(s): 金森涼太、西塚 誠、落合なつき、長田茂宏、今川正良
  • Organizer: 日本薬学会第１３５年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-27
• [Presentation] 成熟脂肪細胞におけるCa2+活性化カリウムチャネルKCNMA1の役割 (2015)
  • Author(s): 西塚 誠、堀之内渉、山田絵理、長田茂宏、今川正良
  • Organizer: 日本薬学会第１３５年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-26
• [Presentation] 脂肪細胞分化促進因子fad24の筋再生における機能解析 (2015)
  • Author(s): 落合なつき、西塚 誠、長田茂宏、今川正良
  • Organizer: 日本薬学会第１３５年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-26
• [Presentation] 脂肪細胞分化制御因子fad104のがん細胞の浸潤・転移における役割 (2014)
  • Author(s): 加藤大輝、西塚 誠、長田茂宏、今川正良
  • Organizer: 第13回次世代を担う若手ファーマ・バイオフォーラム2014
  • Place of Presentation: 富山
  • Year and Date: 2014-09-20
• [Presentation] Fad104, a regulatory factor of adipogenesis, negatively regulates invasion and metastasis of cancer cells (2014)
  • Author(s): Daiki Katoh, Makoto Nishizuka, Shigehiro Osada, Masayoshi Imagawa
  • Organizer: FEBS EMBO 2014 Conference
  • Place of Presentation: パリ
  • Year and Date: 2014-09-02
• [Presentation] 脂肪細胞分化促進因子fad24は胚盤胞形成に必須な役割を担う (2014)
  • Author(s): 落合なつき、長田茂宏、西塚 誠、今川正良
  • Organizer: 日本生化学会中部支部 例会・シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2014-05-24
• [Presentation] 脂肪細胞分化制御因子fad104はがん細胞の浸潤・転移を抑制する (2014)
  • Author(s): 加藤大輝、西塚 誠、長田茂宏、今川正良
  • Organizer: 第15回Pharmaco-hematologyシンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2014-05-24