Functional analysis of lipo genes, PHGPx depleted novel cell death execution factors, identified by genome-wide shRNA library
Project/Area Number |
26460075
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Kitasato University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 細胞死 / 脂質酸化 / 遊離二価鉄 / shRNAライブラリー / フェロトーシス / GPx4欠損細胞死 / リポキシトーシス / ビタミンE / GPx4 / 脂質酸化依存的新規細胞死 / Lipo遺伝子 / GPx4 / タモキシフェン / GFP / 細胞内局在 / PHGPx / GPx4 |
Outline of Final Research Achievements |
To clarify the mechanism of PHGPx depleted novel cell death, we identified six PHGPx depleted lipid peroxidation dependent cell death (lipoxytosis) inducing genes (Lipo gene) by genome-wide shRNA library screening and cell death recovery screening by retransfection of cDNA for lipo gene into lipo gene knockdown cells. Knockdown cells of the Lipo gene failed to suppress ferroptosis by the anti-cancer agent erastin and RSL3. These results reveal that PHGPx-depleted cell death is different cell death pathway from ferroptosis by erastin and RSL3.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] Role of GPx4 in human vascular endothelial cells, and the compensatory activity of brown rice on GPx4 ablation condition.2017
Author(s)
Sakai O,, Yasuzawa T,, Sumikawa Y,, Ueta T,, Imai H,, Sawabe A,, Ueshima S.
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Journal Title
Pathophysiology
Volume: 24(1)
Issue: 1
Pages: 9-15
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Role of Glutathione Peroxidase 4 in Glutamate-induced Oxytosis in the Retina.2015
Author(s)
Sakai, O., Uchida, T., Roggia, MF., Imai, H., Ueta, T., Amano, S.
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Journal Title
Related Report
Peer Reviewed / Open Access
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