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KSHV K-RTA-mediated regulation of immunosuppressive cytokine

Research Project

Project/Area Number 26460076
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionKeio University

Principal Investigator

NOGUHI Kohji  慶應義塾大学, 薬学部(芝共立), 准教授 (80291136)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsウイルス / 転写因子 / サイトカイン / インターロイキン / KSHV / インターロイキン10 / IL-10
Outline of Final Research Achievements

In this study, we have investigated molecular mechanism involved in KSHV K-RTA-mediated regulation of IL-10. At the specific region on IL-10 gene promoter 256 base upstream from first ATG codon, we identified K-RTA-responsive region, and in addition, we found that some host cell factor(s) seemed to modulate K-RTA-mediated IL-10 promoter activation. We found when K-RTA was co-expressed with some transcriptional factor, synergistic activation of IL-10 promoter was induced, and interestingly, posttranslational modification including protein acetylation had some impact on K-RTA. For protein acetylation modification, there are many members of histone acetylase and deacetylase families known to regulate various transcriptional mechanism so that we would like to determine which molecules were involved in K-RTA-mediated IL-10 promoter activation.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (14 results)

All 2017 2016 2015 2014 Other

All Journal Article (7 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 3 results,  Open Access: 2 results) Presentation (5 results) Remarks (2 results)

  • [Journal Article] Novel Mechanisms of Resistance to Investigational Molecularly Targeted Drugs2017

    • Author(s)
      Noguchi K.
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 137 Issue: 2 Pages: 151-160

    • DOI

      10.1248/yakushi.16-00229-6

    • NAID

      130005305801

    • ISSN
      0031-6903, 1347-5231
    • Year and Date
      2017-02-01
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Pharmaceutical Sciences Advancing Molecular Cancer Therapeutics<br/>&#xFF5E;To Improve Rationale and Effectiveness of Molecularly Targeted Drugs&#xFF5E;2017

    • Author(s)
      NOGUCHI K, MASHIMA T
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 137 Issue: 2 Pages: 127-128

    • DOI

      10.1248/yakushi.16-00229-F

    • NAID

      130005305798

    • ISSN
      0031-6903, 1347-5231
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Functional Effects of AKT3 on Aurora Kinase Inhibitor-induced Aneuploidy.2017

    • Author(s)
      Noguchi K, Hongama K, Hariki S, Nonomiya Y, Katayama K, Sugimoto Y.
    • Journal Title

      Journal of Biological Chemistry.

      Volume: 292(5) Issue: 5 Pages: 1910-1924

    • DOI

      10.1074/jbc.m116.747048

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] RSK1 protects P-glycoprotein/ABCB1 against ubiquitin-proteasomal degradation by downregulating the ubiquitin-conjugating enzyme E2 R1.2016

    • Author(s)
      Katayama K, Fujiwara C, Noguchi K, Sugimoto Y.
    • Journal Title

      Scientific Reports.

      Volume: 6 Issue: 1 Pages: 36134-36134

    • DOI

      10.1038/srep36134

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of AKT3 expression on MYC- and caspase-8-dependent apoptosis caused by polo-like kinase inhibitors in HCT 116 cells.2016

    • Author(s)
      Nonomiya Y, Noguchi K, Tanaka N, Kasagaki T, Katayama K, Sugimoto Y.
    • Journal Title

      Cancer Science.

      Volume: 107(12) Issue: 12 Pages: 1877-1887

    • DOI

      10.1111/cas.13093

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Upregulation of cellular glutathione levels in human ABCB5- and murine Abcb5-transfected cells.2015

    • Author(s)
      Kondo S, Hongama K, Hanaya K, Yoshida R, Kawanobe T, Katayama K, Noguchi K, Sugimoto Y
    • Journal Title

      BMC Pharmacol Toxicol.

      Volume: 16 Issue: 1 Pages: 37-37

    • DOI

      10.1186/s40360-015-0038-5

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulations of P-Glycoprotein/ABCB1/MDR1 in Human Cancer Cells2015

    • Author(s)
      Katayama k, Noguchi K, Sugimoto Y.
    • Journal Title

      New Journal of Science

      Volume: 2014 Pages: 476974-476974

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] KSHV RTAのIL-10プロモーター活性化におけるSp3結合領域の役割.2017

    • Author(s)
      宮澤雅典, 野口耕司, 片山和浩, 杉本芳一.
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      東北大学(宮城県・仙台市)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] RTAによるIL-10プロモーター活性化に働く協調分子の同定.2016

    • Author(s)
      宮澤雅典, 野口耕司, 片山和浩, 杉本芳一.
    • Organizer
      第60回日本薬学会関東支部大会
    • Place of Presentation
      東京大学(文京区・東京都)
    • Year and Date
      2016-09-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] KSHV RTAによるinterleukin-10プロモーターの活性化2016

    • Author(s)
      宮澤雅典, 野口耕司, 片山和浩, 杉本芳一
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] KSHV由来RTAに応答するヒトIL-10遺伝子プロモーター領域の解析2015

    • Author(s)
      野口耕司、片山和浩、杉本芳一
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] KSHV由来RTA/ORF50によるIL-10プロモーター活性化2014

    • Author(s)
      野口耕司、片山和浩、杉本芳一
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-11
    • Related Report
      2014 Research-status Report
  • [Remarks] がん分子標的治療薬における薬剤耐性問題の克服と次世代がん薬物治療戦略への応用

    • URL

      https://www.youtube.com/watch?v=AgmSFTnZHo4&t

    • Related Report
      2016 Annual Research Report
  • [Remarks] 慶應義塾 研究者情報データベース

    • URL

      http://www.k-ris.keio.ac.jp/

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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