| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, we have investigated molecular mechanism involved in KSHV K-RTA-mediated regulation of IL-10. At the specific region on IL-10 gene promoter 256 base upstream from first ATG codon, we identified K-RTA-responsive region, and in addition, we found that some host cell factor(s) seemed to modulate K-RTA-mediated IL-10 promoter activation. We found when K-RTA was co-expressed with some transcriptional factor, synergistic activation of IL-10 promoter was induced, and interestingly, posttranslational modification including protein acetylation had some impact on K-RTA. For protein acetylation modification, there are many members of histone acetylase and deacetylase families known to regulate various transcriptional mechanism so that we would like to determine which molecules were involved in K-RTA-mediated IL-10 promoter activation.
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