Mechanisms of remodeling of white adipocytes to brown-like adipocytes (browning) in human mesenchymal stem cells.

Research Project

Project/Area Number	26460077
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Biological pharmacy
Research Institution	Kinjo Gakuin University (2016) Showa University (2014-2015)
Principal Investigator	YAMAGUCHI Tomohiro 金城学院大学, 薬学部, 教授 (50347530)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	脂質代謝 / 脂肪滴 / 脂肪細胞
Outline of Final Research Achievements	Appearance of brown-like adipocytes within white adipose tissue depots (browning) is supposed to be a new target in anti-obesity therapy. The aim of this study was to assess the browning effect of various compounds such as fatty acids, polyphenols, FGF21 and irisin in human adipose-derived mesenchymal stem cells (hADSC). I found that irisin and FGF21 enhanced expression of BAT marker UCP1 and decreased levels of a lipid droplet-associated protein, perilipin1. In further, FGF21 induced levels of perilipin2, suggesting the remodeling from large lipid droplets to smaller droplets.