| Project/Area Number |
26460081
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kyoto Pharmaceutical University |
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Principal Investigator |
Kuga Takahisa 京都薬科大学, 薬学部, 助教 (20551857)
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| Co-Investigator(Kenkyū-buntansha) |
中山 祐治 京都薬科大学, 薬学部, 教授 (10280918)
朝長 毅 国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 プロテオームリサーチプロジェクト, プロジェクトリーダー (80227644)
齊藤 洋平 京都薬科大学, 薬学部, 助教 (90411032)
佐々木 光穂 国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 疾患モデル小動物研究室, プロジェクト研究員 (20432536)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | エナメル質形成不全症 / FAM83H / ケラチン / エナメル質 / 大腸がん / ケラチン骨格 / カゼインキナーゼ1 / 歯 / カゼインキナーゼI / デスモソーム |
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| Outline of Final Research Achievements |
FAM83H plays an essential role in the formation of dental enamel and its heterozygous mutation causes autosomal-dominant hypocalcified amelogenesis imperfecta. We have demonstrated that FAM83H regulates the organization of the keratin cytoskeleton in human colorectal cancer cells. In the present study, we showed that the keratin cytoskeleton in cultured ameloblastoma cells is also regulated by FAM83H. Mutant proteins of FAM83H prevented proper organization of the keratin cytoskeleton and concomitantly dis-localized component proteins of desmosomes form the cell-cell interface in cultured ameloblastoma cells. We additionally generated mice with a mutation in the FAM83H gene. We are testing whether the FAM83H mutation causes the disorganization of the keratin cytoskeleton and desmosomes in dental ameloblasts in vivo.
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