Generation and characterization of brain- specific UBIAD1-deficient mice using a nestin
| Project/Area Number |
26460085
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ビタミンK / UBIAD1 / メナキノン-4 / Nestin-cre / 脳神経 / コンディショナルノックアウトマウス / Nestin / ノックアウトマウス / vitamin K / MK-4 / brain / Menaquinone-4 / コンディショナルノックアウト |
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| Outline of Final Research Achievements |
In this study, we created Nestin-cre Ubiad1-CKO mice which were Ubiad1 deficient from the time of neural stem cell. Nestin-cre Ubiad1-CKO was born normally, but these mice were slightly smaller than that of the wild type mice. We measured the Ubiad1 expression and MK-4 concentration in brain tissue at 10 weeks of age. Ubiad1 expression was remarkably low or below the detection limit in any brain tissues. MK-4 concentration was also remarkably low or below the detection limit. We evaluated the brain functions by behavior analysis for 9 to 10 week old Nestin-cre Ubiad1-CKO mice. As a result, short-term memory, anxiety and motor coordination was decreased in Nestin-cre Ubiad1-CKO mice. Moreover, the brain tissue of Nestin-cre Ubiad1-CKO mouse revealed cerebellar atrophy and disappearance of cerebellar Purkinje cells. Therefore, it was suggested that UBIAD1 and MK-4 play an essential role in maintaining structural and function in the cerebellar.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Vitamin K2 biosynthetic enzyme, UBIAD1 is essential for embryonic development of mice.2014
Author(s)
Nakagawa K, Sawada N, Hirota Y, Uchino Y, Suhara Y, Hasegawa T, Amizuka N, Okamoto T, Tsugawa N, Kamao M, Funahashi N, Okano T
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Journal Title
PLoS ONE.
Volume: 15;9(8)
Issue: 8
Pages: e104078-e104078
DOI
Related Report
Peer Reviewed / Open Access
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