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Generation and characterization of brain- specific UBIAD1-deficient mice using a nestin

Research Project

Project/Area Number 26460085
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionKobe Pharmaceutical University

Principal Investigator

Nakagawa Kimie  神戸薬科大学, 薬学部, 准教授 (90309435)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsビタミンK / UBIAD1 / メナキノン-4 / Nestin-cre / 脳神経 / コンディショナルノックアウトマウス / Nestin / ノックアウトマウス / vitamin K / MK-4 / brain / Menaquinone-4 / コンディショナルノックアウト
Outline of Final Research Achievements

In this study, we created Nestin-cre Ubiad1-CKO mice which were Ubiad1 deficient from the time of neural stem cell. Nestin-cre Ubiad1-CKO was born normally, but these mice were slightly smaller than that of the wild type mice. We measured the Ubiad1 expression and MK-4 concentration in brain tissue at 10 weeks of age. Ubiad1 expression was remarkably low or below the detection limit in any brain tissues. MK-4 concentration was also remarkably low or below the detection limit. We evaluated the brain functions by behavior analysis for 9 to 10 week old Nestin-cre Ubiad1-CKO mice. As a result, short-term memory, anxiety and motor coordination was decreased in Nestin-cre Ubiad1-CKO mice. Moreover, the brain tissue of Nestin-cre Ubiad1-CKO mouse revealed cerebellar atrophy and disappearance of cerebellar Purkinje cells. Therefore, it was suggested that UBIAD1 and MK-4 play an essential role in maintaining structural and function in the cerebellar.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] YY1 positively regulates human UBIAD1 expression2015

    • Author(s)
      Funahashi N, Hirota Y, Nakagawa K, Sawada N, Watanabe M, Suhara Y, Okano T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: in press Issue: 2 Pages: 238-244

    • DOI

      10.1016/j.bbrc.2015.03.018

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Vitamin K2 biosynthetic enzyme, UBIAD1 is essential for embryonic development of mice.2014

    • Author(s)
      Nakagawa K, Sawada N, Hirota Y, Uchino Y, Suhara Y, Hasegawa T, Amizuka N, Okamoto T, Tsugawa N, Kamao M, Funahashi N, Okano T
    • Journal Title

      PLoS ONE.

      Volume: 15;9(8) Issue: 8 Pages: e104078-e104078

    • DOI

      10.1371/journal.pone.0104078

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 脳神経特異的ビタミンK2合成酵素UBIAD1欠損マウスの表現型解析2017

    • Author(s)
      中川公恵、原 香織、三宅智子、泰井麻由奈、横田衣利、澤田夏美、須原義智、長谷川 潤、岡野登志夫
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] ビタミンKの新たな機能探索~ビタミンK2合成酵素UBIAD1欠損マウスの解析から~2017

    • Author(s)
      中川公恵
    • Organizer
      埼玉医科大学ゲノム医学研究センター運営員会承認学術集会
    • Place of Presentation
      埼玉
    • Year and Date
      2017-03-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脳神経特異的ビタミンK2合成酵素UBIAD1欠損マウスの脳機能解析2016

    • Author(s)
      中川公恵、泰井麻由奈、横田衣利、原 香織、三宅智子、澤田夏美、須原義智、岡野登志夫、長谷川 潤
    • Organizer
      日本ビタミン学会第69回大会
    • Place of Presentation
      富山
    • Year and Date
      2016-06-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脳神経系におけるビタミンK2合成酵素UBIAD1の機能解析2016

    • Author(s)
      中川公恵、澤田夏美、須原義智、岡野登志夫
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-29
    • Related Report
      2015 Research-status Report
  • [Presentation] UBIAD1 knockout mice for insights into the elucidation of MK-4 function in brain2015

    • Author(s)
      中川公恵
    • Organizer
      FASEB Conference Molecular, Structural & Clinical Aspects of Vitamin K and Vitamin K-Dependent Proteins
    • Place of Presentation
      Itasca, Illinois (USA)
    • Year and Date
      2015-07-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ビタミンK2合成酵素Ubiad1遺伝子欠損マウスの表現型解析2015

    • Author(s)
      中川公恵、澤田夏美、廣田佳久、内野由理、須原義智、岡野登志夫
    • Organizer
      日本ビタミン学会第67回大会
    • Place of Presentation
      奈良
    • Year and Date
      2015-06-06
    • Related Report
      2014 Research-status Report
  • [Presentation] ビタミンK2合成酵素Ubiad1遺伝子欠損マウスは胎生致死となる2015

    • Author(s)
      中川公恵、澤田夏美、廣田佳久、内野由理、須原義智、岡野登志夫
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Generation and characterization of brain-specific UBIAD1-deficient mice using a nestin promoter-driven cre/loxP strategy2014

    • Author(s)
      Kimie Nakagawa
    • Organizer
      4th International Conference on Clinical & Experimental Ophthalmology
    • Place of Presentation
      Baltimore
    • Year and Date
      2014-07-14 – 2014-07-16
    • Related Report
      2014 Research-status Report
    • Invited
  • [Book] ビタミンKと疾患-基礎の理解と臨床への応用2014

    • Author(s)
      中川公恵
    • Publisher
      医薬ジャーナル社
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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