Melanogenesis regulation by expression and localization of copper-transporters.
Project/Area Number |
26460086
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Nagasaki International University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | チロシナーゼ / 銅輸送体 / 銅シャペロン / メラノソーム / リソソーム / ユビキチン / ユビキチンリガーゼ / タンパク質分解 |
Outline of Final Research Achievements |
We have examined a possibility that an activity and expression of tyrosinase, a copper binding proteina and key enzyme of melanin synthesis, is regulated by an intracellular level of copper ion. We found that RNF152, an ubiquitin ligase, specifically interacts with and ubiquitinates tyrosinase. Since RNF152 dose not interacts with and ubiquitinates other melanin synthesis enzyme, Tyrp-1, we concluded that RNF152 may be involved in the regulation of melanin synthesis by controlling an expression level and intracellular localization of tyrosinase in melanocytes. We are unable to show the data that copper-transporters regulate an expression level and intracellular localization of tyrosinase in melanocytes.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Internalization of a novel, huge lectin from Ibacus novemdentatus (slipper lobster) induces apoptosis of mammalian cancer cells.2017
Author(s)
Fujii Y, Fujiwara T, Koide Y, Hasan I, Sugawara S, Rajia S, Kawsar SM, Yamamoto D, Araki D, Kanaly RA, Ogawa Y, Fujita H, Ozeki Y.
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Journal Title
Glycoconj J
Volume: 34
Issue: 1
Pages: 85-94
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Inulavosin and its benzo-derivatives, melanogenesis inhibitors, target the copper loading mechanism to the active site of tyrosinase.2014
Author(s)
Fujita H., Menezes J.C., Santos S.M., Yokota S., Kamat S.P., Cavaleiro J.A., Motokawa T., Kato T., Mochizuki M., Fujiwara T., Fujii Y., Tanaka Y.
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Journal Title
Pigment Cell Melanoma Res.
Volume: 27
Issue: 3
Pages: 376-386
DOI
Related Report
Peer Reviewed
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[Presentation] Inulavosin and its benzo-derivatives affect on copper-loading mechanism to tyrosinase, a key enzyme of melanin synthesis in melanocytes2014
Author(s)
H. Fujita,.J.M.D.S. Menezes, S. M. Santos, S. Yokota, S. P. Kamat, J. A.S. Cavaleiro, T. Motokawa, T. Kato, M. Mochizuki, Y. Fujii and T. Fujiwara
Organizer
第22回国際色素細胞学会
Place of Presentation
Singapore
Year and Date
2014-09-05
Related Report
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