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Melanogenesis regulation by expression and localization of copper-transporters.

Research Project

Project/Area Number 26460086
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionNagasaki International University

Principal Investigator

Fujita Hideaki  長崎国際大学, 薬学部, 教授 (80291524)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsチロシナーゼ / 銅輸送体 / 銅シャペロン / メラノソーム / リソソーム / ユビキチン / ユビキチンリガーゼ / タンパク質分解
Outline of Final Research Achievements

We have examined a possibility that an activity and expression of tyrosinase, a copper binding proteina and key enzyme of melanin synthesis, is regulated by an intracellular level of copper ion. We found that RNF152, an ubiquitin ligase, specifically interacts with and ubiquitinates tyrosinase. Since RNF152 dose not interacts with and ubiquitinates other melanin synthesis enzyme, Tyrp-1, we concluded that RNF152 may be involved in the regulation of melanin synthesis by controlling an expression level and intracellular localization of tyrosinase in melanocytes. We are unable to show the data that copper-transporters regulate an expression level and intracellular localization of tyrosinase in melanocytes.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 2014

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 4 results,  Open Access: 1 results) Presentation (3 results) Book (1 results)

  • [Journal Article] Synthesis of β-tubulin occurs within chromatoid body of round spermatids.2017

    • Author(s)
      Fujii Y, Fujita H, Yokota S
    • Journal Title

      Cytoskeleton

      Volume: 74 Issue: 5 Pages: 197-204

    • DOI

      10.1002/cm.21363

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Internalization of a novel, huge lectin from Ibacus novemdentatus (slipper lobster) induces apoptosis of mammalian cancer cells.2017

    • Author(s)
      Fujii Y, Fujiwara T, Koide Y, Hasan I, Sugawara S, Rajia S, Kawsar SM, Yamamoto D, Araki D, Kanaly RA, Ogawa Y, Fujita H, Ozeki Y.
    • Journal Title

      Glycoconj J

      Volume: 34 Issue: 1 Pages: 85-94

    • DOI

      10.1007/s10719-016-9731-x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Argonaute2 protein in rat spermatogenic cells is localized to nuage structures and LAMP2-positive vesicles surrounding chromatoid bodies.2016

    • Author(s)
      Fujii Y, Onohara Y, Fujita H, and Yokota S
    • Journal Title

      Journal of Histochemistry & Cytochemistry

      Volume: 64 Pages: 268-279

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Membrane-associated RING-CH (MARCH) 8 inhibits HIV-1 infection by reducing virion incorporation of envelope glycoproteins2015

    • Author(s)
      Tada T, Zhang Y, Koyama T, Tobiume M, Tsunetsugu-Yokota Y, Yamaoka S, Fujita H and Tokunaga K
    • Journal Title

      Nature Medicine

      Volume: 21 Pages: 1502-1507

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Inulavosin and its benzo-derivatives, melanogenesis inhibitors, target the copper loading mechanism to the active site of tyrosinase.2014

    • Author(s)
      Fujita H., Menezes J.C., Santos S.M., Yokota S., Kamat S.P., Cavaleiro J.A., Motokawa T., Kato T., Mochizuki M., Fujiwara T., Fujii Y., Tanaka Y.
    • Journal Title

      Pigment Cell Melanoma Res.

      Volume: 27 Issue: 3 Pages: 376-386

    • DOI

      10.1111/pcmr.12225

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 膜結合型ユビキチンリガーゼRNF152は後期エンドソームにおいてチロシナーゼと相互作用・共局在する2016

    • Author(s)
      高橋紫蓉、濱美月、藤井佑樹、Jose C.J.M.D.S. Menezes、高橋宏隆、竹田 浩之、澤崎 達也、本川 智紀、徳永 研三、藤田 英明
    • Organizer
      日本色素細胞学会
    • Place of Presentation
      岐阜
    • Year and Date
      2016-11-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] 美白剤イヌラボシンが標的とするチロシナーゼ特異的銅シャペロンの同定2015

    • Author(s)
      藤田英明
    • Organizer
      第26回 日本色素細胞学会
    • Place of Presentation
      札幌
    • Year and Date
      2015-11-14
    • Related Report
      2015 Research-status Report
  • [Presentation] Inulavosin and its benzo-derivatives affect on copper-loading mechanism to tyrosinase, a key enzyme of melanin synthesis in melanocytes2014

    • Author(s)
      H. Fujita,.J.M.D.S. Menezes, S. M. Santos, S. Yokota, S. P. Kamat, J. A.S. Cavaleiro, T. Motokawa, T. Kato, M. Mochizuki, Y. Fujii and T. Fujiwara
    • Organizer
      第22回国際色素細胞学会
    • Place of Presentation
      Singapore
    • Year and Date
      2014-09-05
    • Related Report
      2014 Research-status Report
  • [Book] 新しい機能形態学-ヒトの成り立ちとその働き 第3版2015

    • Author(s)
      伊藤萌子、稲葉二朗、江川祥子、岡美佳子、籠田智美、川井範夫、木澤靖夫、黒岩美枝、小崎康子、佐々木啓子、篠塚和正、清水典史、高橋知子、竹鼻眞、馬場広子、藤田英明、益子崇、松岡耕二、森山賢治、山口宣秀
    • Total Pages
      470
    • Publisher
      廣川書店
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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