| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
GEPRA is the genetically encoded probe for retinoic acid. Because a high dose uptake of the retinoic acid results in teratogenesis in vertebrates, it is crucial to know how the retinoic acid distribute in embryos. Although we have successfully visualized the retinoic acid distribution in zebrafish, GEPRA had a tendency to form aggregates in mammalian cells when overly expressed. Furthermore, it is not bright because GEPRA utilizes a dim fluorescent protein.
To overcome the drawbacks, we extensively engineered the probe. Finally we have obtained a brighter GEPRA without aggregates (we named the improved GEPRA, mGEPRA). We further succeeded in establishing transgenic mouse lines expressing mGEPRA.
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