Generation of new drug seed natural products from entomopathogenic microorganism by activating silent biosynthetic gene clusters
| Project/Area Number |
26460117
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Natural medicines
|
|---|
| Research Institution | The University of Tokyo (2015-2016)
Tohoku University (2014) |
|---|
Principal Investigator |
ASAI Teigo 東京大学, 大学院総合文化研究科, 准教授 (60572310)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | 天然物化学 / 二次代謝物 / 糸状菌 / 休眠遺伝子 / 薬理活性天然物 / 二次代謝活性化 / リボゾーム工学 / 生合成 / 活性天然物 / 活性化 / 薬理活性物質 / 天然物 / 昆虫病原性微生物 / ケミカルエピジェネティクス / ランダム変異 |
|---|
| Outline of Final Research Achievements |
In this study, we aimed to develop several methods for activating silent biosynthetic gene cluster to find a variety of novel natural products and biological active compounds to be drug seeds. First we made mutants with hygromycin B resistance of various fungi by using chemical mutagentic approach and evaluated their ability of secondary metabolite production. From the results, we found that the use of mutants with hygromycin B resistance is significantly effective to stimuli fungal silent biosynthetic pathways. Next, we screened a large number of entomopathogenic fungi and fungi living in insects by applying the newly developped method in addition to chemical epigenetics and successfully obtained a diverse novel natural products.
|
Report
(4 results)
Research Products
(26 results)
-
-
-
-
-
[Journal Article] Use of a Biosynthetic Intermediate to Explore the Chemical Diversity of Pseudo-natural Fungal Polyketides2015
Author(s)
T. Asai*, K. Tsukada, S. Ise, N. Shirata, M. Hashimoto, I. Fujii, K. Gomi, K. Nakagawara, E. N. Kodama, Y, Oshima
-
Journal Title
Nature Chem.
Volume: 7
Issue: 9
Pages: 737-743
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
