| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
In this study, we aimed to develop several methods for activating silent biosynthetic gene cluster to find a variety of novel natural products and biological active compounds to be drug seeds. First we made mutants with hygromycin B resistance of various fungi by using chemical mutagentic approach and evaluated their ability of secondary metabolite production. From the results, we found that the use of mutants with hygromycin B resistance is significantly effective to stimuli fungal silent biosynthetic pathways. Next, we screened a large number of entomopathogenic fungi and fungi living in insects by applying the newly developped method in addition to chemical epigenetics and successfully obtained a diverse novel natural products.
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