Elucidation of the mechanism of the component changes during processing of safflower flowers and the chemical structure of the components after the change

• Project/Area Number: 26460120
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Kumamoto University (2018-2019); Nagoya University (2017); University of Toyama (2014-2016)
• Principal Investigator: Kazuma Kohei 熊本大学, 大学院生命科学研究部附属グローバル天然物科学研究センター, 特任准教授 (70552446)
• Project Period (FY): 2014-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2018: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2017: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: ベニバナ色素 / 乱花 / べに餅 / LC－MS分析 / ケモメトリクス / 単離構造解析 / カーサミン / キノカルコン類 / QCGアルデヒド / プレカーサミン / 紅 / ベニバナ

Outline of Final Research Achievements

In this study, a typical processed safflower flower product was prepared using the traditional method that was actually used. The components contained in the obtained product were analyzed by LC-MS, and then those data were evaluated statistically. As is known, the difference between sun-dried flowers and fermented flowers was that the content of Beni (carthamin) was high in red rice cake. It was revealed for the first time that it would be done.
It was difficult to isolate these components using only the molecular weight as an index. Therefore, LC-MS analysis was conducted focusing on the decomposed product of carthamin, and three characteristic components were detected. Since the same components were confirmed in the processed product extract, they were isolated and structurally analyzed. As a result, QCG-aldehyde and safflor-metabolin were identified.

Academic Significance and Societal Importance of the Research Achievements

本研究は、ベニバナ花に関して伝統産業で実際に行われている複数の加工に着目し、それらの製品に含まれる成分の差異を明らかにしたことに意義がある。ベニバナ花の加工法に科学的説明を加えることを通じて、ベニバナの生産と６次産業化、伝統産業、生薬国内生産の発展を後押しするものである。

Research Products

• [Presentation] ベニバナ花のトランスクリプトーム解析とカルコン合成酵素のクローニングおよび機能解析 (2016)
  • Author(s): 篠崎淳一，兼目裕充，二瓶賢一，野路征昭，紺野勝弘，浅川義範，数馬恒平
  • Organizer: 天然有機化合物討論会
  • Place of Presentation: 東北大学
  • Year and Date: 2016-09-14
• [Presentation] An LC-MS Study on Agricultural Products of the Flowers of Safflower. (2016)
  • Author(s): Kohei Kazuma
  • Organizer: The International Symposium on Natural Products for the Future 2016
  • Place of Presentation: 徳島文理大学
  • Year and Date: 2016-08-31
• [Presentation] ベニバナ花加工品の成分 (2016)
  • Author(s): 数馬恒平・紺野勝弘
  • Organizer: 日本薬学会135回年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-03-26
• [Presentation] Gene Mining for Carthamin Biosynthesis in Safflower, Carthamus tinctorius L., Using Next-generation Sequencing Platforms (2015)
  • Author(s): Kenmoku, H., Shinozaki, J., Masuda, K., Noji, M., Konno, K., Asakawa, Y., Kazuma, K.
  • Organizer: Inaugural Symposium of the Phytochemical Society of Asia 2015 Tokushima
  • Place of Presentation: 徳島文理大学
  • Year and Date: 2015-08-30
  • Int'l Joint Research
• [Book] 油用作物ベニバナの葉も食用となる（おもしろサイエンス 機能性野菜の科学（佐竹元吉編）中で） (2016)
  • Author(s): 数馬恒平
  • Total Pages: 2
  • Publisher: 日刊工業新聞社