| Project/Area Number |
26460139
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
Itoh Kouichi 徳島文理大学, 薬学部, 教授 (30291149)
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| Co-Investigator(Kenkyū-buntansha) |
石原 康宏 広島大学, 総合科学研究科, 助教 (80435073)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 漢方薬 / てんかん / 脳浮腫 / BBB / 脳内炎症 / 予防 / 五苓散 / 血管原性脳浮腫 / 側頭葉てんかんモデルマウス / MRI / てんかん原性 |
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| Outline of Final Research Achievements |
In the present study, we determined that treatment with Kampo Gorei-san (GRS) after status epilepticus (SE) termination by diazepam might prevent the neurotoxicity induced by brain edema events via blood-brain barrier (BBB), and the possible molecular and cellular mechanisms of GRS treatment. To assess the effect of GRS against the brain alterations after SE, we focused on BBB dysfunction associated with angiogenesis and brain inflammation. The consecutive treatment of GRS inhibited the temporarily increased BBB leakage in the hippocampus two days after SE. These findings suggested that these brain alterations by SE-induced BBB failure were prevented by GRS treatment. Furthermore, in the acute phase of the latent period, these upregulations of pro-inflammatory-related molecules were inhibited by GRS treatment. These findings suggest that GRS is likely involved in neuroprotection via anti-inflammatory activities against BBB dysfunction in the acute phase of epileptogenesis after SE.
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