Regulation of subcellular localization and functions of proteins by in-cell catalytic reactions
Project/Area Number |
26460142
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Drug development chemistry
|
Research Institution | The University of Tokyo |
Principal Investigator |
Yamatsugu Kenzo 東京大学, 大学院薬学系研究科(薬学部), 助教 (30646807)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | in-cell reaction / subcellular localization / protein / catalyst / acylation / 細胞内反応 / 細胞内局在 / タンパク質 / 触媒 / アシル化 / タンパク質化学修飾 |
Outline of Final Research Achievements |
This research aimed at regulating subcellular localization of proteins through their catalytic chemical modifications in living cells as a new method for functional regulations of the proteins. I succeeded in developing a novel catalyst system to enable protein-selective acylation, and I found that a protein modified by the catalyst system (fatty acylation) localized in liposomes. I, furthermore, succeeded in protein-selective acylation in living cells using the catalyst system. Although I, unfortunately, have not achieved subcellular localization control in living cells, I believe that this research achievement paved a way for a new method of regulating protein functions through a novel mechanism.
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Report
(5 results)
Research Products
(3 results)