| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We aimed to build a foundation for the next-generation of oligonucleotides drug including antigene technology by means of syntheses and evaluations of conformationally-restricted nucleosides. In this study, first, we synthesized novel nucleosides having a C4'-endo type sugar moiety or C3-endo type one. As a result of evaluating the triple-stranded oligonucleotides' stability, it was revealed that 2'-5' linked nucleic acid restricted to a C3-endo conformation, which was believed to destabilize the higher-order structure so far, can stably form a triplex did. Moreover, we clarified that the rotation angle around the glycosidic bond is a major factor determining the stability of the higher order structure by comparing the structure details of these artificial oligonucleotides with LNAs.
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