Study on development and application of histochemically stainable probe using fluorescent pigment dye
| Project/Area Number |
26460166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Drug development chemistry
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| Research Institution | Hiroshima International University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | シアリダーゼ / 蛍光イメージング / シアル酸 / 組織染色 / インフルエンザ / ライブイメージング / 蛍光色素 / ケミカルバイオロジー / 蛍光 / イメージング / 酵素活性 / 可視化 / 蛍光基質 / グルコシダーゼ |
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| Outline of Final Research Achievements |
In this study, we developed the novel artificial sialidase substrate with 2-benzothiazol-2-yl-phenol skeltone, which is the fluorescent pigment dye.
It was revealed that the influenza infected cells had been histochemically stained with the developed sialidase substrate, and the substrate could detect the infection of not only the human influrenza virus but also the avian influenza virus, non-traditonal type (H1N1) or drug-resistant viruses. And it was easily possible to detecte the drug-resistant virusese by additon of the novel sialidase substrate and anti-influenza-neuraminidase drug in a few minute. The drug resistance property was indicated by a fluorescent focus formed by an activity of the neuraminidase, so the unknown mutation resulting the new drug resistant viruse would be detected.
The developed novel substrate is now commercially available under the support of WAKO JUNYAKU KOGYO.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Substrate Specificity of Equine and Human Influenza A Virus Sialidase to Molecular Species of Sialic Acid2016
Author(s)
Tadanobu Takahashi, Saori Unuma, Sawako Kawagishi, Yuuki Kurebayashi, Maiko Takano, Hiroki Yoshino, Akira Minami, Takashi Yamanaka, Tadamune Otsubo, Kiyoshi Ikeda, Takashi Suzuki.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 39
Issue: 10
Pages: 1728-1733
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] High-efficiency capture of drug resistant-influenza virus by live imaging of sialidase activity2016
Author(s)
Yuuki Kurebayashi, Tadanobu Takahashi, Chihiro Tamoto, Keiji Sahara, Tadamune Otsubo, Tatsuya Yokozawa, Nona Shibahara, Hirohisa Wada, Akira Minami, Kiyoshi Ikeda, Takashi Suzuki
Organizer
Sialoglyco 2016
Place of Presentation
The Fess Parker - A Doubletree
Year and Date
2016-11-14
Related Report
Int'l Joint Research
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