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Study on development and application of histochemically stainable probe using fluorescent pigment dye

Research Project

Project/Area Number 26460166
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Drug development chemistry
Research InstitutionHiroshima International University

Principal Investigator

Otsubo Tadamune  広島国際大学, 薬学部, 准教授 (30365879)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsシアリダーゼ / 蛍光イメージング / シアル酸 / 組織染色 / インフルエンザ / ライブイメージング / 蛍光色素 / ケミカルバイオロジー / 蛍光 / イメージング / 酵素活性 / 可視化 / 蛍光基質 / グルコシダーゼ
Outline of Final Research Achievements

In this study, we developed the novel artificial sialidase substrate with 2-benzothiazol-2-yl-phenol skeltone, which is the fluorescent pigment dye.
It was revealed that the influenza infected cells had been histochemically stained with the developed sialidase substrate, and the substrate could detect the infection of not only the human influrenza virus but also the avian influenza virus, non-traditonal type (H1N1) or drug-resistant viruses. And it was easily possible to detecte the drug-resistant virusese by additon of the novel sialidase substrate and anti-influenza-neuraminidase drug in a few minute. The drug resistance property was indicated by a fluorescent focus formed by an activity of the neuraminidase, so the unknown mutation resulting the new drug resistant viruse would be detected.
The developed novel substrate is now commercially available under the support of WAKO JUNYAKU KOGYO.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (10 results) (of which Int'l Joint Research: 1 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Role of sialidase in long-term potentiation at mossy fiber-CA3 synapses and hippocampus-dependent spatial memory.2016

    • Author(s)
      Akira Minami, Masakazu Saito, Shou Mamada, Daisuke Ieno, Tomoya Hikita, Tadanobu Takahashi, Tadamune Otsubo, Kiyoshi Ikeda and Takashi Suzuki.
    • Journal Title

      PLoS One

      Volume: 11 Issue: 5 Pages: e0156400-e0156400

    • DOI

      10.1371/journal.pone.0156400

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Substrate Specificity of Equine and Human Influenza A Virus Sialidase to Molecular Species of Sialic Acid2016

    • Author(s)
      Tadanobu Takahashi, Saori Unuma, Sawako Kawagishi, Yuuki Kurebayashi, Maiko Takano, Hiroki Yoshino, Akira Minami, Takashi Yamanaka, Tadamune Otsubo, Kiyoshi Ikeda, Takashi Suzuki.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 39 Issue: 10 Pages: 1728-1733

    • DOI

      10.1248/bpb.b16-00345

    • NAID

      130005598489

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Role of sialidase in long-term potentiation at mossy fiber-CA3 synapses and hippocampus-dependent spatial memory2016

    • Author(s)
      Akira Minami, Masakazu Saito, Shou Mamada, Daisuke Ieno, Tomoya Hikita, Tadanobu Takahashi, Tadamune Otsubo, Kiyoshi Ikeda and Takashi Suzuki
    • Journal Title

      PLOS ONE

      Volume: 11

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 老化脳に蓄積する希少シアル酸分子種は海馬依存性記憶を障害する2017

    • Author(s)
      横山喬太、南彰、山下千晶、田口理紗、大坪忠宗、池田 潔、海野けい子、武田厚司、鈴木隆
    • Organizer
      第137回日本薬学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒトパラインフルエンザウイルス検出のための新規蛍光イメージング剤の開発研究2017

    • Author(s)
      福間彩加、山口千尋、大坪忠宗、寺岡文照、紅林佑希、南彰、高橋忠伸、鈴木隆、池田潔
    • Organizer
      第137回日本薬学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 定着性を向上させたシアリダーゼプローブの合成2017

    • Author(s)
      大坪忠宗、紅林佑希、高橋忠伸、南彰、鈴木隆、寺岡文照、池田潔
    • Organizer
      第137回日本薬学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] High-efficiency capture of drug resistant-influenza virus by live imaging of sialidase activity2016

    • Author(s)
      Yuuki Kurebayashi, Tadanobu Takahashi, Chihiro Tamoto, Keiji Sahara, Tadamune Otsubo, Tatsuya Yokozawa, Nona Shibahara, Hirohisa Wada, Akira Minami, Kiyoshi Ikeda, Takashi Suzuki
    • Organizer
      Sialoglyco 2016
    • Place of Presentation
      The Fess Parker - A Doubletree
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ライブイメージングを指向した蛍光シアリダーゼ基質の合成と応用2016

    • Author(s)
      大坪 忠宗、紅林 佑希、寺岡 文照、南 彰、高橋 忠伸、鈴木 隆、池田 潔
    • Organizer
      第42回反応と合成の進歩シンポジウム
    • Place of Presentation
      静岡市清水文化会館マリナート
    • Year and Date
      2016-11-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] 組織染色可能な蛍光シアリダーゼ基質の合成と応用2016

    • Author(s)
      大坪 忠宗、紅林 佑希、寺岡 文照、南 彰、高橋 忠伸、鈴木 隆、池田 潔
    • Organizer
      第35回糖質学会
    • Place of Presentation
      高知市文化プラザ かるぽーと
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] 薬剤耐性の検出にも利用可能なインフルエンザウイルス蛍光検出法の確立2016

    • Author(s)
      紅林佑希、高橋忠伸、大坪忠宗、南彰、池田潔、鈴木隆
    • Organizer
      第30回インフルエンザ研究者交流の会シンポジウム
    • Place of Presentation
      山形市保健センター
    • Year and Date
      2016-06-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] 高感度なβ-グルコシダーゼイメージング剤の合成と蛍光組織染色への応用2016

    • Author(s)
      大坪忠宗、石井亜実、南彰、高橋忠伸、鈴木隆、寺岡文照、池田潔
    • Organizer
      日本薬学会136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 異なる起源を持つグルコシダーゼの特異性定量化法の開発2015

    • Author(s)
      大坪忠宗、南彰、安藤究、田口理沙、高橋忠伸、鈴木隆、寺岡文照、池田潔
    • Organizer
      第134回日本薬学会総会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 蛍光性顔料色素を用いたシアリダーゼ基質の合成とシアリダーゼ活性の可視化2014

    • Author(s)
      大坪 忠宗、南 彰、高橋 忠伸、金澤 寛明、鈴木 隆、寺岡 文照、池田 潔
    • Organizer
      日本ケミカルバイオロジー学会 第9回年会
    • Place of Presentation
      大阪
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Research-status Report
  • [Book] BIO INDUSTRY2016

    • Author(s)
      大坪忠宗、池田潔、紅林佑希、南彰、高橋忠伸、鈴木隆
    • Total Pages
      10
    • Publisher
      シーエムシー出版
    • Related Report
      2016 Annual Research Report
  • [Remarks] 蛍光性顔料色素を用いたバイオイメージング剤の開発

    • URL

      http://www.hirokoku-u.ac.jp/assets/files/cooperation/seeds/yootubo.pdf

    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 新規化合物及び該化合物を含む蛍光組成物2015

    • Inventor(s)
      鈴木隆、高橋忠伸、南彰、池田潔、大坪忠宗
    • Industrial Property Rights Holder
      鈴木隆、高橋忠伸、南彰、池田潔、大坪忠宗
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-018871
    • Filing Date
      2015-02-02
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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