| Project/Area Number |
26460167
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Drug development chemistry
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| Research Institution | Kitakyushu National College of Technology |
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Principal Investigator |
Inoue Yuichi 北九州工業高等専門学校, 生産デザイン工学科, 准教授 (20284911)
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| Co-Investigator(Kenkyū-buntansha) |
川原 浩治 北九州工業高等専門学校, 生産デザイン工学科, 教授 (20321515)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | ヒト細胞株 / 医療用タンパク質 / GMP |
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| Outline of Final Research Achievements |
To establish new GMP-compliant human cell lines for therapeutic protein production, we determined the conditions for making immortalized cells from human peripheral blood mononuclear cells in a non-GMP environment. Then, we improved them for a GMP environment and succeeded in obtaining human B lymphoid clones that could be cultured for a long time. The obtained clones might be mainly suitable for secreted protein production because mature B lymphocytes have the extensive endoplasmic reticulum. Further study on serum-free cell culture and cell protein productivity enhancement will lead to a practical application of therapeutic protein production using human cells.
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