Creation of a low toxic derivative biosynthesis system for biologically active substances derived from bacteria such as endotoxin
| Project/Area Number |
26460179
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Doshisha Women's College of Liberal Arts |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | エンドトキシン / 脂質 / 質量分析 / 菌体成分 / 免疫刺激 |
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| Outline of Final Research Achievements |
Bacteria contain components that activate the human immune system. The analog can be a pharmaceutical seed. It is effective to create a system in bacteria by modifying the biosynthesis / metabolic system. Achromobacter xylosoxidans has ornithine-containing lipids. Adjuvant activity is known for amino acid-containing lipids including ornithine-containing lipids. Therefore, an analytical method using a matrix-assisted laser desorption ionization time-of-flight mass spectrometer of amino acid-containing lipids was established. Detection and purification of hydroxylated ornithine-containing lipids were carried out using the analytical method. The bioactivity of hydroxylated ornithine-containing lipids and nonhydroxylated ornithine-containing lipids was compared, but there was no difference as far as it was investigated.
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Report
(5 results)
Research Products
(5 results)
