• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Generation of vascular smooth muscle specific Ubiad1 gene knockout mice and phenotype analysis

Research Project

Project/Area Number 26460180
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental and hygienic pharmacy
Research InstitutionOsaka Shoin Women's University (2015-2016)
Kobe Pharmaceutical University (2014)

Principal Investigator

Tsugawa Naoko  大阪樟蔭女子大学, 健康栄養学部, 教授 (30207352)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsビタミンK / UBIAD1 / 血管特異的遺伝子欠損 / 血管平滑筋 / Transgelin-Cre / メナキノン4 / 血管石灰化
Outline of Final Research Achievements

UbiA prenyltransferase domain containing 1 (UBIAD1) is a novel vitamin K2 biosynthetic enzyme. To investigate the function of UBIAD1 in vascular smooth muscle cells, we attempted to generate vascular specific conditional Ubiad1 knockout mice (Ubiad1-V-KO). Ubiad1-V-KO was generated with conditional deletion of Ubiad1 gene in cells expressing the smooth muscle cell-specific protein SM22α. Ubiad1-V-KO were born according to Mendel's theory of heredity, however all Ubiad1-V-KO were died with pleural effusion at 6-8 weeks-old. Also, we confirmed that abnormality of aortic elastic fiber, ventricular myocardial hypoplasia, hypertension and decrease of heart rate in Ubiad1-V-KO.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015 2014

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Nongenomic effects of 1α,25-dihydroxyvitamin D3 on cartilage formation deduced from comparisons between Cyp27b1 and Vdr knockout mice2017

    • Author(s)
      Y Hirota K Nakagawa S Mimatsu N Sawada T Sakaki N Kubodera M Kamao N Tsugawa Y Suhara T Okano
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 483 Issue: 1 Pages: 359-365

    • DOI

      10.1016/j.bbrc.2016.12.139

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Association between vitamin D status and serum parathyroid hormone concentration and calcaneal stiffness in Japanese adolescents: sex differences in susceptibility to vitamin D deficiency.2016

    • Author(s)
      Tsugawa N, Uenishi K, Ishida H, Ozaki R, Takase T, Minekami T, Uchino Y, Kamao M, Okano T.
    • Journal Title

      J Bone Miner Metab.

      Volume: 34

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of the vitamin K2 biosynthetic enzyme UBIAD1.2015

    • Author(s)
      Hirota Y, Nakagawa K, Sawada N, Okuda N, Suhara Y, Uchino Y, Kimoto T, Funahashi N, Kamao M, Tsugawa N, Okano T.
    • Journal Title

      PLoS One.

      Volume: 10

    • NAID

      130007413127

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cardiovascular Diseases and Fat Soluble Vitamins: Vitamin D and Vitamin K.2015

    • Author(s)
      Tsugawa N
    • Journal Title

      J Nutr Sci Vitaminol (Tokyo).

      Volume: 61 Pages: 170-172

    • NAID

      130005109894

    • Related Report
      2015 Research-status Report
  • [Journal Article] Effects of Fok-I polymorphism in vitamin D receptor gene on serum 25-hydroxyvitamin D, bone-specific alkaline phosphatase and calcaneal quantitative ultrasound parameters in young adults.2015

    • Author(s)
      Tanabe R, Kawamura Y, Tsugawa N, Haraikawa M, Sogabe N, Okano T, Hosoi T, Goseki-Sone M.
    • Journal Title

      Asia Pac J Clin Nutr.

      Volume: 24 Pages: 329-335

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fibroblast Growth Factor-23 and Vitamin D Metabolism in Subjects with eGFR >=60 ml/min/1.73 m2.2015

    • Author(s)
      Nakatani S, Nakatani A, Tsugawa N, Yamada S, Mori K, Imanishi Y, Ishimura E, Okano T, Inaba M.
    • Journal Title

      Nephron.

      Volume: 130 Pages: 119-126

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] High prevalence of vitamin D deficiency in patients with xeroderma pigmetosum-A under strict sun protection.2015

    • Author(s)
      Kuwabara A, Tsugawa N, Tanaka K, Uejima Y, Ogawa J, Otao N, Yamada N, Masaki T, Nishigori C, Moriwaki S, Okano T.
    • Journal Title

      Eur J Clin Nutr. 2015

      Volume: 69 Pages: 693-696

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional Characterization of the Vitamin K2 Biosynthetic Enzyme UBIAD1.2015

    • Author(s)
      Hirota Y, Nakagawa K, Sawada N, Okuda N, Suhara Y, Uchino Y, Kimoto T, Funahashi N, Kamao M, Tsugawa N, Okano T.
    • Journal Title

      PLoS One

      Volume: 10

    • NAID

      130007413127

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vitamin K2 biosynthetic enzyme, UBIAD1 is essential for embryonic development of mice.2014

    • Author(s)
      Nakagawa K, Sawada N, Hirota Y, Uchino Y, Suhara Y, Hasegawa T, Amizuka N, Okamoto T, Tsugawa N, Kamao M, Funahashi N, Okano T.
    • Journal Title

      PLoS One

      Volume: 9

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] The Elevation of Renal Renin Production Does Not Necessarily Correlate with Blood Pressure in Vitamin D Receptor Gene Knockout Mice Fed Hypocalcemia Rescue Diet.2015

    • Author(s)
      Naoko Tsugawa, Miku Wada, Kisato Kanao, Toshio Okano
    • Organizer
      2015 Annual Meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Seattle, USA
    • Year and Date
      2015-10-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 異所性石灰化に伴う軟組織における骨芽細胞様形質の獲得とMatrix Gla Protein(MGP)発現の関係2015

    • Author(s)
      苫田紘佳、津川尚子、中村 拳、山本真由美、安田真央、中川公恵、岡野登志夫
    • Organizer
      日本ビタミン学会第67回大会
    • Place of Presentation
      奈良
    • Year and Date
      2015-06-05
    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi