| Project/Area Number |
26460181
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
見明 史雄 福岡大学, 薬学部, 教授 (50248522)
佐藤 朝光 福岡大学, 薬学部, 助教 (90369025)
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| Research Collaborator |
Hiramatsu Yukihiro 福岡大学, 大学院・薬学研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 乳酸菌 / ゲノムDNA / 抗炎症作用 / オリゴデオキシヌクレオチド / TLR９ / Hsp90 / Hsp70 / TLR9 / NF-κβ / TLR-9 / Elafin / Lactobacillus plantarum / 抗炎症 / DNA / 炎症性腸疾患 / ODN |
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| Outline of Final Research Achievements |
We identified 14 novel anti-inflammatory oligodeoxynucleotide (ODN) from the genomic DNA of Lactobacillus casei using Caco-2 cells. In particular, the ODN 7F (TTTTGCCG) also showed the anti-inflammatory effects in THP-1 cells used as immune cell model and oral administration of 7F ameliorated the severity of colitis in dextran sodium sulfate-induced mouse model of IBD. In addition, the anti-inflammatory effects of 7F were mainly regulated by an increase in heat shock protein (Hsp) 70 expression through TLR9 and Hsp90. Our results suggested that the anti-inflammatory effects of live LAB is dependent on their genomic DNA and ODN identified from LAB genomic DNA possess the potential for treating IBD.
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