Development of cell microarray chip for rapid and high sensitive malaria diagnosis in clinical fields
| Project/Area Number |
26460186
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
YATSUSHIRO Shouki 国立研究開発法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (90399155)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 感染症 / マラリア原虫 / 細胞チップ / 薬学 / マラリア |
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| Outline of Final Research Achievements |
The infection rates were 0.08 to 23.6 % by Giemsa staining. On the other hand, the infection rates were estimated as 0.08 to 2.53 % by cell microarray chip system. Although we could not detect good correlation between two methods above 2.0 % (Fig. 3), good correlation was observed according to simple linear regression analysis (R2=0.9222) below the 1.8 %. This cell microarray chip system was developed for the high sensitive detection of malaria infected erythrocytes (100 times higher sensitivity than that of conventional light microscopy),
Although cell microarray chip system could not detect malaria infected erythrocytes exactly (above 2.0 %), accurate detection could be performed in low infection rate (below 1.8 %). Furthermore, the number of patients was not enough to demonstrate the potential of cell microarray chip system for diagnosis system. We will improve the cell microarray chip system for field setting with more number of malaria patient’s samples.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Absence of in vivo selection for K13 mutations after artemether-lumefantrine treatment in Uganda.2017
Author(s)
Balikagala B, Mita T, Ikeda M, Sakurai M, Yatsushiro S, Takahashi N, Tachibana SI, Auma M, Ntege EH, Ito D, Takashima E, Palacpac NM, Egwang TG, Onen JO, Kataoka M, Kimura E, Horii T, Tsuboi T.
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Journal Title
Malar J.
Volume: 16(1)
Issue: 1
Pages: 1-23
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Prognostic Impact of Circulating Tumor Cell Detected Using a Novel Fluidic Cell Microarray Chip System in Patients with Breast Cancer.2016
Author(s)
Sawada T, Araki J, Yamashita T, Masubuchi M, Chiyoda T, Yunokawa M, Hoshi K, Tao S, Yamamura S, Yatsushiro S, Abe K, Kataoka M, Shimoyama T, Maeda Y, Kuroi K, Tamura K, Sawazumi T, Minami H, Suda Y, Koizumi F.
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Journal Title
EBioMedicine.
Volume: 11
Pages: 173-182
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Application of a cell microarray chip system for accurate, highly sensitive, and rapid diagnosis for malaria in Uganda.2016
Author(s)
Yatsushiro S, Yamamoto T, Yamamura S, Abe K, Obana E, Nogami T, Hayashi T, Sesei T, Oka H, Okello-Onen J, Odongo-Aginya EI, Alai MA, Olia A, Anywar D, Sakurai M, Palacpac NM, Mita T, Horii T, Baba Y, Kataoka M.
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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