The development of novel biomarker for arteriosclerosis using circulating miRNAs.
| Project/Area Number |
26460203
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
Iwaki Soihiro 名古屋市立大学, 大学院薬学研究科, 講師 (60399962)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | miRNA / バイオマーカー / 動脈硬化 / エクソソーム |
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| Outline of Final Research Achievements |
To identify the novel miRNA markers for arteriosclerosis, we screened total RNA samples derived from patient's plasma using a microarray platform. We found that several circulating miRNAs were differentially expressed. We also demonstrated that expression of miRNAs was altered by various cytokine that induced the inflammation state in the human umbilical cord vein endothelial cells (HUVECs). Furthermore, we investigated the optimal RNA extraction method of miRNA from plasma samples for quantitative PCR. These results suggest that circulating miRNAs are available as a novel biomarker to assess the arteriosclerosis.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Increased Acid Ceramidase Expression depends on Upregulation of Androgen-dependent Deubiquitinases, USP2, in a Human Prostate Cancer Cell Line, LNCaP.2015
Author(s)
Naoki Mizutani, Minami Inoue, Yukari Omori, Hiromi Ito, Keiko Tamiya-Koizumi, Akira Takagi, Tetsuhito Kojima, Mitsuhiro Nakamura, Soichiro Iwaki, Masahiro Nakatochi, Motoshi Suzuki, Yoshinori Nozawa, Takashi Murate
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Journal Title
J. Biochem.
Volume: 印刷中
Issue: 4
Pages: 309-319
DOI
Related Report
Peer Reviewed
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[Journal Article] Increased Plasma Sphingosine-1-Phosphate in Obese Subjects and Its Capacity to Increase Expression of Plasminogen Activator Inhibitor-1 in Adipocytes2013
Author(s)
Shiori Ito, Soichiro Iwaki, Keiko Koike, Yuichiro Yuda, Ayako Nagasaki, Ryunosuke Ohkawa, Yutaka Yatomi, Tomoo Furumoto, Hiroyuki Tsutsui, Burton E Sobel, Satoshi Fujii
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Journal Title
Coron. Artery Dis
Volume: 24
Issue: 8
Pages: 642-650
DOI
Related Report
Peer Reviewed
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