The development of novel biomarker for arteriosclerosis using circulating miRNAs.

• Project/Area Number: 26460203
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: Iwaki Soihiro 名古屋市立大学, 大学院薬学研究科, 講師 (60399962)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: miRNA / バイオマーカー / 動脈硬化 / エクソソーム

Outline of Final Research Achievements

To identify the novel miRNA markers for arteriosclerosis, we screened total RNA samples derived from patient's plasma using a microarray platform. We found that several circulating miRNAs were differentially expressed. We also demonstrated that expression of miRNAs was altered by various cytokine that induced the inflammation state in the human umbilical cord vein endothelial cells (HUVECs). Furthermore, we investigated the optimal RNA extraction method of miRNA from plasma samples for quantitative PCR. These results suggest that circulating miRNAs are available as a novel biomarker to assess the arteriosclerosis.

Research Products

• [Journal Article] Sphingosine 1-phosphate induced by hypoxia increases the expression of PAI-1 in HepG2 cells via HIF-1α (2016)
  • Author(s): Akimasa Sanagawa, Soichiro Iwaki, Moyoko Asai, Daisuke Sakakibara, Hiroaki Norimoto, Burton E. Sobel, Satoshi Fujii
  • Journal Title: Mol Med Rep. Volume: 14 Issue: 2 Pages: 1841-1848
  • DOI: 10.3892/mmr.2016.5451
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Targeting ceramide synthase 6-dependent metastasis-prone phenotype in lung cancer cells. (2016)
  • Author(s): Suzuki M, et al.
  • Journal Title: J. Clin. Invest. Volume: 126 Issue: 1 Pages: 254-265
  • DOI: 10.1172/jci79775
  • Peer Reviewed / Open Access
• [Journal Article] Circulating Level of MicroRNA-126 may be a Potential Biomarker for Recovery from Smoking-Related Vascular Damage in Middle-Aged Habitual Smokers. (2015)
  • Author(s): Tomonori Sugiura, Yasuaki Dohi, Sumiyo Yamashita, Soichiro Iwaki, Shiori Ito, Akimasa Sanagawa, Nobuyuki Ohte, Satoshi Fujii
  • Journal Title: IJC Heart & Vasculature Volume: 印刷中 Pages: 83-87
  • DOI: 10.1016/j.ijcha.2015.02.012
  • Peer Reviewed / Open Access
• [Journal Article] Increased Acid Ceramidase Expression depends on Upregulation of Androgen-dependent Deubiquitinases, USP2, in a Human Prostate Cancer Cell Line, LNCaP. (2015)
  • Author(s): Naoki Mizutani, Minami Inoue, Yukari Omori, Hiromi Ito, Keiko Tamiya-Koizumi, Akira Takagi, Tetsuhito Kojima, Mitsuhiro Nakamura, Soichiro Iwaki, Masahiro Nakatochi, Motoshi Suzuki, Yoshinori Nozawa, Takashi Murate
  • Journal Title: J. Biochem. Volume: 印刷中 Issue: 4 Pages: 309-319
  • DOI: 10.1093/jb/mvv039
  • Peer Reviewed
• [Journal Article] Increased Plasma Sphingosine-1-Phosphate in Obese Subjects and Its Capacity to Increase Expression of Plasminogen Activator Inhibitor-1 in Adipocytes (2013)
  • Author(s): Shiori Ito, Soichiro Iwaki, Keiko Koike, Yuichiro Yuda, Ayako Nagasaki, Ryunosuke Ohkawa, Yutaka Yatomi, Tomoo Furumoto, Hiroyuki Tsutsui, Burton E Sobel, Satoshi Fujii
  • Journal Title: Coron. Artery Dis Volume: 24 Issue: 8 Pages: 642-650
  • DOI: 10.1097/mca.0000000000000033
  • Peer Reviewed
• [Presentation] The role of the HIF-1α/HuR pathway in increased expression of PAI-1 induced by sphingosine 1-phosphate in HepG2 cells rendered hypoxic (2016)
  • Author(s): Satoshi Fujii, Akimasa Sanagawa, Soichiro Iwaki, Moyoko Asai, Daisuke Sakakibara, Hiroaki Norimoto
  • Organizer: XXlllrd International Congress on Fibrinolysis & Proteolysis and XVlth International Workshop on Molecular and Cellular Biology of Plasminogen Activation
  • Place of Presentation: 静岡市
  • Int'l Joint Research
• [Presentation] 低酸素環境が血管内皮細胞由来miRNAと血管内皮機能に与える影響の解析 (2015)
  • Author(s): 藤井 聡, 小笠原美沙, 岩城壮一郎
  • Organizer: 第37回日本血栓止血学会学術集会
  • Place of Presentation: 甲府市総合市民会館
  • Year and Date: 2015-05-22
• [Presentation] Blood Pressure Reduction and Increase of Circulating MicroRNA-126 Indicate Improvement of Vascular Endothelial Damage After Smoking Cessation Intervention in Middle-Aged Habitual Smokers (2014)
  • Author(s): Tomonori Sugiura, Yasuaki Dohi, Sumiyo Yamashita, Akimasa Sanagawa, Soichiro Iwaki, Nobuyuki Ohte, Satoshi Fujii
  • Organizer: American Heart Association's 2014 Scientific Sessions and Resuscitation Science Symposium
  • Place of Presentation: シカゴ、アメリカ
  • Year and Date: 2014-11-18
• [Presentation] 転写因子CREBによるSphingosine kinase 2発現調節機序 (2014)
  • Author(s): 水谷直貴、大森由佳里、小泉恵子、鈴木 元、高木 明、小嶋哲人、岩城壮一郎、藤井 聡、中村光浩、野澤義則、村手 隆
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2014-10-16
• [Presentation] NKTハイブリドーマにおけるGLP-1によるS1Pを介したTNF-α発現制御機構 (2014)
  • Author(s): 長谷川諒、伊藤史織、岩城壮一郎、岩渕和也、佐藤雅、藤井聡
  • Organizer: 第36回日本血栓止血学会学術集会
  • Place of Presentation: 大阪国際交流センター
  • Year and Date: 2014-05-31
• [Presentation] スフィンゴシン1 リン酸/アポリポタンパクA1 含有金ナノ粒子による炎症性動脈硬化に対する新規ドラッグデリバリーシステムの開発 (2014)
  • Author(s): 田上辰秋、高橋朋弘、岩城壮一郎、藤井 聡、尾関哲也
  • Organizer: 第15回Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2014-05-24
• [Presentation] スフィンゴ脂質が線溶系に与える影響の分子機構 (2014)
  • Author(s): 岩城壮一郎
  • Organizer: 第15回Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2014-05-23
  • Invited