Effect of hyperhomocysteinemia on vascular dysfunction in renal failure
| Project/Area Number |
26460207
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ICHIDA Kimiyoshi 東京薬科大学, 薬学部, 教授 (80183169)
NAKAMURA Makiko 東京薬科大学, 薬学部, 助教 (80447557)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ホモシステイン / メチオニン / 腎不全 / 代謝フラックス / GC-MS / 安定同位体 / 血管内皮 / 血管平滑筋 / 一炭素転移 / 動脈硬化 / 代謝回転 / 1炭素転移 / メチル基転移 |
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| Outline of Final Research Achievements |
Chronic kidney disease (CKD) and hyperhomocysteinemia are risk factors of cardiovascular disease individually. While the association of CKD and hyperhomocysteinemia is well established in clinical populations, little is known about the role of hyperhomocysteinemia in the development of vascular dysfunction of CKD. This study is conducted to clarify how plasma homocysteine (Hcy) is elevated in CKD and the effects of hyperhomocysteinemia on vascular function in CKD. We determined the turn over rates for Hcy production and disposal of 5/6 nephrectomized rats using a stable isotope technique and showed that decreased remethylation of Hcy to methionine (Met) was associated with hyperhomocysteinemia in CKD. Using 5/6 nephrectomized rats with hyperhomocysteinemia induced by Met-enriched diets, vasodilataions of thoracic aorta were determined. Combination of hyperhomocysteinemia and CKD resulted in impairments of both endothelium-dependent and endothelium-independent vasodilatations.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Hypoxanthine guanine phosphoribosyltransferase (HPRT) deficiencies: HPRT1 mutations in new Japanese families and PRPP concentration.2014
Author(s)
Yasukazu Yamada, Noriko Nomura, Kenichiro Yamada, Reiko Kimura, Daisuke Fukushi, Noubuaki Wakamatsu, Yasufumi Matsuda, Takahiro Yamauchi, Takanori Ueda, Hiroshi Hasegawa, Makiko Nakamura, Kimiyoshi Ichida, Kiyoko Kaneko, Shin Fujimori.
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Journal Title
Nucleosides, Nucleotides, Nucleic Acids
Volume: 33
Pages: 218-222
Related Report
Peer Reviewed
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[Journal Article] Hydrophilic-interaction liquid chromatography–tandem mass spectrometric determination of erythrocyte 5-phosphoribosyl 1-pyrophosphate in patients with hypoxanthine–guanine phosphoribosyltransferase deficiency2014
Author(s)
Hiroshi Hasegawa, Yoshihiko Shinohara, Sayako Nozaki, Makiko Nakamura, Koei Oh, Osamu Namiki, Kiyotaka Suzuki, Akihiko Nakahara, Mari Miyazawa, Ken Ishikawa, Takahiro Himeno, Sayaka Yoshida, Takanori Ueda, Yasukazu Yamada, Kimiyoshi Ichida
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Journal Title
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Volume: 976-977
Pages: 55-60
DOI
Related Report
Peer Reviewed
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[Presentation] Plasma kinetics of D-serine in rats.2014
Author(s)
Hiroshi Hasegawa, Yoshihiko Shinohara, Nami Masuda, Takumi Natori, Kimiyoshi Ichida.
Organizer
The 2nd International Conference of D-Amino Acid Research
Place of Presentation
栃木、宇都宮
Year and Date
2014-09-02 – 2014-09-05
Related Report
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