• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of the molecular mechanism of unstirred water layer

Research Project

Project/Area Number 26460208
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Medical pharmacy
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

KISHIMOTO Hisanao  東京薬科大学, 薬学部, 助教 (80723600)

Co-Investigator(Kenkyū-buntansha) 井上 勝央  東京薬科大学, 薬学部, 教授 (50315892)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords消化管吸収 / 非攪拌水層 / 粘液層 / 吸収促進 / 一酸化窒素 / 脂溶性薬物 / mucin / 吸収促進剤 / 受動拡散
Outline of Final Research Achievements

In the intestinal absorption of lipophilic drugs mediated by simple diffusion, the rate-limiting step has been considered to be the drug permeation process through an unstirred water layer (UWL). Although UWL may play a role in intestinal drug absorption, the contribution remains unknown at the molecular level. On the other hand, nitric oxide (NO) has been known to increase the intestinal permeability of water-soluble compounds by modulating paracellular pathway. However, it is unclear whether NO can enhance the absorption of lipophilic drugs through the transcellular pathway. In this research project, we demonstrated the contribution of the UWL to the absorption of lipophilic drugs, and that the effect of UWL on intestinal absorption is dependent on the intestinal regions, where the expression of mucin molecules may be regulated. Furthermore, we found that NO has the capability to enhance the intestinal absorption of lipophilic drugs by modulating the UWL function.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (10 results) (of which Invited: 1 results)

  • [Journal Article] Kishimoto H, Miyazaki K, Takizawa Y, Shirasaka Y, Inoue K2016

    • Author(s)
      Absorption-enhancing effect of nitric oxide on the absorption of hydrophobic drugs in rat duodenum.
    • Journal Title

      J Pharm Sci

      Volume: 105 Issue: 2 Pages: 729-733

    • DOI

      10.1002/jps.24677

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 脂溶性薬物の腸管膜透過性に及ぼす腸管粘液の影響及び mucin 発現解析2017

    • Author(s)
      宮崎 歌織、岸本 久直、村谷 美穂、田野 文音、白坂 善之、井上 勝央
    • Organizer
      日本薬剤学会第32年会
    • Place of Presentation
      大宮ソニックシティ(大宮)
    • Year and Date
      2017-05-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] ラット腸管における粘液層を介した脂溶性薬物の吸収に対する一酸化窒素の影響2016

    • Author(s)
      宮崎歌織
    • Organizer
      日本薬物動態学会第31回年会
    • Place of Presentation
      キッセイ文化ホール(長野県松本文化会館)/ 松本市総合体育館(松本)
    • Year and Date
      2016-10-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] Effects of Mucosal Protective Agents on the Expression Levels of Mucus Layer Components in Human Intestinal Cell Lines2016

    • Author(s)
      H. Kishimoto, N. Murata, M. Muratani, K. Miyazaki, Y. Shirasaka and K. Inoue.
    • Organizer
      日本薬物動態学会第31回年会
    • Place of Presentation
      キッセイ文化ホール(長野県松本文化会館)/ 松本市総合体育館(松本)
    • Year and Date
      2016-10-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] 一酸化窒素による難吸収性薬物の腸管促進効果 に関する研究 に関する研究 に関する研究2016

    • Author(s)
      岸本久直
    • Organizer
      第33回製剤設計研究会
    • Place of Presentation
      東京薬科大学(東京)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 経粘膜透過を介した薬物の腸管吸収に及ぼす粘液層の影響2016

    • Author(s)
      宮崎歌織、岸本久直、村谷美穂、白坂善之、井上勝央
    • Organizer
      日本薬剤学会第31年会
    • Place of Presentation
      長良川国際会議場(岐阜)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 経粘膜透過を介した薬物の腸管吸収に及ぼす粘液層の影響2016

    • Author(s)
      宮崎歌織、岸本久直、村谷美穂、白坂善之、井上勝央
    • Organizer
      日本薬剤学会 第31年会
    • Place of Presentation
      長良川国際会議場(岐阜)
    • Year and Date
      2016-05-19
    • Related Report
      2015 Research-status Report
  • [Presentation] 岸本久直、宮崎歌織、村谷美穂、瀧沢裕輔、白坂善之、井上勝央2016

    • Author(s)
      脂溶性薬物の腸管吸収に与える粘液層の影響
    • Organizer
      日本薬学会 第136年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] Effect of nitric oxide on the intestinal absorption of lipophilic drugs in rat2015

    • Author(s)
      Hisanao Kishimoto, Kaori Miyazaki, Yusuke Takizawa, Yoshiyuki Shirasaka, Katsuhisa Inoue
    • Organizer
      日本薬物動態学会 第30回年会
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2015-11-12
    • Related Report
      2015 Research-status Report
  • [Presentation] 経細胞輸送を介した薬物の消化管吸収に及ぼす一酸化窒素の影響2015

    • Author(s)
      宮崎 歌織、岸本 久直、瀧沢 裕輔、白坂 善之、井上 勝央
    • Organizer
      日本薬剤学会第30年会
    • Place of Presentation
      長崎ブリックホール
    • Year and Date
      2015-05-21 – 2015-05-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 経細胞輸送を介した薬物の消化管吸収に及ぼす一酸化窒素の影響2015

    • Author(s)
      宮崎歌織、岸本久直、瀧沢裕輔、白坂善之、井上勝央
    • Organizer
      日本薬剤学会 第30年会
    • Place of Presentation
      長崎ブリックホール(長崎)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi