Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Outline of Final Research Achievements |
Profile determination methods for oxysterols sulfate and tetrahydrocorticosteroids glucuronides (THC-G) and/or these sulfates in urine samples by means of LC/MS/MS were developed. The method was applicable to quantitative analysis of THC-G in dried urine spots (DUS) sample. The method was well validated, and the concentration of THC-G in DUS were well correlated with those in liquid urine. DUS samples provides convenient and safe transportation, storage and handling of sample. DUS samples would be a useful alternative for clinical screening tests of endocrine disorders, particularly, when the sample needs to be transported over long distances.
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