| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we performed analysis to identify fatty acid which plays an important role in HIF-1 activation by fatty acid-bearing albumin exposure and to examine an involvement of PPAR gamma in fatty acid-bearing albumin-induced HIF-1 activation in HK-2 cells.
Treatment with palmitic acid and oleic acid had no significant effect on GLUT-mediated transport activity, which is enhanced by HIF-1 activation, while the activity of GLUT was increased by treatment with arachidonic acid. These results indicated that arachidonic acid is involved in albumin-induced HIF-1 activation. We also observed induction of HIF-1 alpha, GLUT1 and BCRP mRNA expression by treatment with a PPAR gamma agonist pioglitazone. In addition, pioglitazone increased transport activity of GLUT and BCRP in a concentration-dependent manner. These findings suggested that PPAR gamma induced HIF-1 activation, resulting in enhanced transporter activity of GLUT and BCRP, HIF-1 target gene products.
|
