Analysis of Caspr in glioma invasion
| Project/Area Number |
26460222
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Kagoshima University |
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Principal Investigator |
Takeda Yasuo 鹿児島大学, 医歯学域附属病院, 教授 (60245462)
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| Co-Investigator(Kenkyū-buntansha) |
池田 龍二 鹿児島大学, 医歯学域附属病院, 准教授 (50398278)
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| Research Collaborator |
FUTAGAWA TOSHITAKA 鹿児島大学, 病院, 薬剤師
TAKAHAMA KAZUHIRO 鹿児島大学, 病院, 薬剤師
MASUDA SHOGO 鹿児島大学, 病院, 薬剤師
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Caspr1 / 膠芽腫 / 移動・浸潤 / JNK / MMP-9 / 神経接着関連因子 / グリオーマ / 浸潤抑制 / Caspr |
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| Outline of Final Research Achievements |
Caspr family molecules are transmembrane glycoproteins of neurexin superfamily which play a role for myelination of axons in brain. Recently, it has been reported about functions of Caspr family molecules in cancer cells. However, it is unclear about roles of Caspr molecules in cancer cells. In this study, we surveyed expression of Caspr in human glioblastoma cell-lines, and found expression of Caspr1. Secondly, we investigated relation of Caspr1 expression levels and cells proliferation, migration, invasion. In the results, suppression of Caspr1 by treatment with Caspr1-targeted siRNA promoted T98G glioblastoma cells invasion, migration and increased MMP-9 expression levels. Moreover, knockdown of Caspr1 increased JNK phosphorylation, and JNK inhibitor blocked MMP-9 up-regulation and cells invasion, migration by knockdown of Caspr1. Thus, these results suggest that Caspr1 negatively regulates for T98G glioblastoma cells invasion and migration via down-regulation of JNK pathway.
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Report
(4 results)
Research Products
(37 results)
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Author(s)
Kawamura H, Matsumoto K, Shigemi A, Orita M, Nakagawa A, Nozima S, Tominaga H, Setoguchi T, Komiya S, Tokuda K, Nishi J
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Journal Title
American Journal of Infection Control
Volume: 44(2)
Pages: 210-214
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[Journal Article] One-year intranasal application of growth hormone releasing peptide-2 improves body weight and hypoglycemia in a severely emaciated anorexia nervosa patient.2015
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Haruta I, Fuku Y, Kinoshita K, Yoneda K, Morinaga A, Amitani M, Amitani H, Asakawa A, Sugawara H, Takeda Y, Bowers CY, Inui A.
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Journal Title
Journal of Cachexia, Sarcopenia and Muscle
Volume: 6
Issue: 3
Pages: 237-241
DOI
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Peer Reviewed / Open Access
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[Journal Article] Trough level monitoring of intravenous busulfan to estimate the area under the plasma drug concentration-time curve in pediatric hematopoietic stem cell transplant recipients.2015
Author(s)
Watanabe E, Nishikawa T, Ikawa K, Yamaguchi H, Abematsu T, Nakagawa S, Kurauchi K, Kodama Y, Tanabe T, Shinkoda Y, Matsumoto K, Okamoto Y, Takeda Y, Kawano Y.
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Journal Title
International Journal of Hematology
Volume: 102
Issue: 5
Pages: 611-616
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Contactin-Associated Protein 4 Interaction with Ligand of Numb Protein X2 Modulates the Proliferation and Neuronal Differentiation of Mouse Neural Progenitor Cells2015
Author(s)
Yin Feng-Ting, Futagawa Toshitaka, Li Di, Ma Yan-Xia, Lu Mei-Hong, Lu Li, Li Shen, Chen Yao, Cao Yong-Jun, Yang Zara Zhuyun, Oiso Shigeru, Nishida Kentaro, Kuchiiwa Satoshi, Watanabe Kazutada, Yamada Katsushi, Takeda Yasuo, Xiao Zhi-Cheng, and Ma Quan-Hong.
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Journal Title
Stem Cells and Development
Volume: 24
Pages: 640-652
Related Report
Peer Reviewed / Open Access
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[Journal Article] Factors Affecting Treatment and Recurrence of Clostridium difficile infections.2014
Author(s)
Kazuaki Matsumoto, Naoko Kanazawa, Akari Shigemi, Kazuro Ikawa, Norifumi Morikawa, Toyoyasu Kooriyama, Michiyo Orita, Hideki Kawamura, Kouichi Tokuda, Junichirou Nishi, Yasuo Takeda.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 37
Pages: 1811-1815
NAID
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[Journal Article] Green tea catechin, epigallocatechin-3-gallate, attenuates the cell viability of human non-small-cell lung cancer A549 cells via reducing Bcl-xL expression2014
Author(s)
Jun-Ichoro Sonoda, Ryuji Ikeda, Yasutaka BabA, Keiko Narumi, Akio Kawachi, Erisa Tomishige, Kazuya Nishihara, Yasuo Takeda, Katsushi Yamada, Keizo Sato, and Toshiro Motoya
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Journal Title
Experimental and Therpeutic Medicine
Volume: 8
Pages: 59-63
Related Report
Peer Reviewed / Open Access
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