Aquaporins involved with diabetes insipidus and the investigation of natural compounds controling the function of aquaporns
| Project/Area Number |
26460223
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大山 雅義 岐阜薬科大学, 薬学部, 教授 (30381718)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | アクアポリン / 細胞膜移行 / 細胞質移行 / プロテインキナーゼＣ / アクチン / 微小管 / Akt / トラフィキング / AQP1 / AQP3 / AQP9 / オイゲノール / レスベラトロール / ヒト前立腺がん細胞 / プロテインキナーゼC / 緑色蛍光タンパク質 / Ａｋｔ |
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| Outline of Final Research Achievements |
Aquaporins which are intrinsic membrane proteins functioning as a water channel permeable to water and glycerol. All aquaporin molecules expressing in cells do not always exist in a cell membrane, but the trafficking of aquaporins in a cell are controlled to move between the cell membrane and inside the cell as necessary. We found that the movement of aquaporin 3 from inside to membrane was regulated by protein kinase C and that from membrane to inside was done by protein kinase B (Akt). Phosphorylation of Thr237 and Ser275 in aquaporin 3 was confirmed to be necessary to move from inside the cell to cell membrane.
We investigated natural compounds which triggered the trafficking of aquaporins. Eugenol which is a component of magnolia flower derived from Magnolia salicifolia was found to promote the trafficking of aquaporin 3 from inside to membrane accompanying the phosphorylation of protein kinase C.
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Report
(5 results)
Research Products
(5 results)
