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Approach for Differentiating Trophoblast Cell Lineage from Human Induced Pluripotent Stem Cells with Retinoic Acid

Research Project

Project/Area Number 26460242
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Medical pharmacy
Research InstitutionOsaka Ohtani University

Principal Investigator

Ikeda Kenji  大阪大谷大学, 薬学部, 准教授 (10434812)

Co-Investigator(Renkei-kenkyūsha) URASHIMA Yoko  大阪大谷大学, 薬学部, 助教 (90636309)
Research Collaborator OGAWA Masafumi  大阪大谷大学, 薬学部, 非常勤講師
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords妊娠時薬物療法 / 胎児移行性 / iPS / 分化 / シンシチオトロホブラスト / 胎盤 / レチノイン酸 / iPS細胞 / トランスポーター / 薬物透過性 / 評価モデル / Drug Placental Transport
Outline of Final Research Achievements

Evaluation of placental drug transport is the first step in chemotherapeutic safety evaluations during pregnancy, but a well-established in vitro model is not available. Therefore, it is necessary to increase the similarities between the syncytiotrophoblast, the main layer of the placental barrier, and the in vitro evaluation model. We focused on the in vivo similarities of differentiating induced pluripotent stem cells (iPSCs). In this study, the conditions required to differentiate iPSCs into syncytiotrophoblasts, such as retinoic acid (RA), a factor required for the differentiation of hematopoietic stem cells from iPSCs, were investigated using hCG secretion as a marker of syncytiotrophoblasts. We demonstrated that iPSCs differentiate into syncytiotrophoblasts, characterized by marked hCG secretion after RA treatment. Our future studies will involve optimization of the differentiation conditions for iPSC-derived syncytiotrophoblast cell layers by RA.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results)

  • [Journal Article] Carrier-Mediated Placental Transport of Cimetidine and Valproic Acid across Differentiating JEG-3 Cell Layers2015

    • Author(s)
      Kenji Ikeda
    • Journal Title

      Pharmazie

      Volume: 70 Pages: 471-476

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] In vitro approach for placental drug transport studies using induced pluripotent stem cells.2017

    • Author(s)
      Kenji Ikeda
    • Organizer
      15th International Congress of Therapeutic Drug Monitoring & Clinical Toxicology (IATDMCT), in Kyoto.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] iPS細胞を用いたin vitro医薬品胎児移行性評価系の構築に関する検討2017

    • Author(s)
      池田賢二
    • Organizer
      第38回白金シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] iPS細胞を用いたin vitro医薬品胎児移行性評価系の構築に関する検討2017

    • Author(s)
      池田 賢二
    • Organizer
      第38回白金シンポジウム
    • Place of Presentation
      北里大学薬学部2202講義室
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 物理化学領域と薬学実務領域との連動に迫る2016

    • Author(s)
      池田 賢二
    • Organizer
      日本薬学会第136年会(横浜)シンポジウム
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] iPS細胞を用いたin vitro 医薬品胎児移行性評価系の確立に関する検討~その2遺伝子解析から~2016

    • Author(s)
      池田 賢二、川崎 由栞、松田 仁美、浦嶋 庸子、廣谷 芳彦
    • Organizer
      第26回日本医療薬学会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2016 Research-status Report
  • [Presentation] iPS細胞を用いたin vitro 医薬品胎児移行性評価系の確立に関する検討~その1機能解析から~2016

    • Author(s)
      川崎 由栞、池田 賢二、松田 仁美、浦嶋 庸子、廣谷 芳彦
    • Organizer
      第26回日本医療薬学会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2016 Research-status Report
  • [Presentation] In vitro Approach for the Placental Drug Transport Evaluation Model Using Induced Pluripotent Stem Cells2015

    • Author(s)
      Kenji Ikeda
    • Organizer
      14th International Congress of Therapeutic Drug Monitoring & Clinical Toxicology (IATDMCT)
    • Place of Presentation
      The conference venue "De Doelen", Rotterdam, Netherlands
    • Year and Date
      2015-10-11
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] In vitro胎盤薬物透過評価系細胞層におけるリアルタイム経上皮電気抵抗値測定2014

    • Author(s)
      池田賢二, 八木裕太, 小倉晴可, 植田ちづる, 川西園代, 初田泰敏, 西井諭司, 小川雅史
    • Organizer
      第31回日本TDM学会
    • Place of Presentation
      ソラシティカンファレンスセンター
    • Year and Date
      2014-05-31 – 2014-06-01
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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