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Analysis for heterosynaptic competition between parallel and climbing fiber in mature Purkinje cells

Research Project

Project/Area Number 26460250
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionHokkaido University

Principal Investigator

Miyazaki Taisuke  北海道大学, 医学研究科, 助教 (90374230)

Research Collaborator YAMASAKI MIWAKO  北海道大学, 医学研究科, 准教授 (10431305)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsプルキンエ細胞 / 登上線維 / 平行線維 / 電位依存性カルシウムチャネル / シナプス競合
Outline of Final Research Achievements

Purkinje cells receive two excitatory inputs, parallel fiber (PF) and climbing fiber (CF). This PC circuitry is established by heterosynaptic competition between PF and CF, fueled by glutamate receptor GluRδ2 and voltage-gated calcium ion channel Cav2.1, respectively. However, it has been unclear whether this heterosynaptic competition maintains PC circuitry in the adult cerebellum. To uncover this question, I investigated novel mouse line, in which Cav2.1 gene can be deleted in adult PCs by the drug-induced ablation system. In anatomical and electrophysiological investigation, inducible Cav2.1 ablation in the adult cerebellum caused motor discoordination and imbalance of heterosynaptic competiton with proximal expansion of PF territory. Therefore, Cav2.1 fuels CF inputs and balance heterosynaptic competition in the adult cerebellum. This result strongly suggests that the balance of heterosynaptic competition is essential for the cerebellar function.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (21 results)

All 2017 2016 2015 2014

All Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Open Access: 9 results,  Acknowledgement Compliant: 8 results) Presentation (8 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Ionic Basis for Membrane Potential Resonance in Neurons of the Inferior Olive2016

    • Author(s)
      Matsumoto-Makidono, Y., Nakayama, H., Yamasaki, M., Miyazaki, T., Kobayashi, K., Watanabe, M., Kano, M., Sakimura, K., Hashimoto, K.
    • Journal Title

      Cell Reports

      Volume: 16 Issue: 4 Pages: 994

    • DOI

      10.1016/j.celrep.2016.06.053

    • URL

      https://pure.teikyo.jp/en/publications/3ce87e4a-7df7-4d28-8c16-070ceff28970

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus.2016

    • Author(s)
      Kobayashi S, Hida Y, Ishizaki H, Inoue E, Tanaka-Okamoto M, Yamasaki M, Miyazaki T, Masahiro F, Kitajima I, Takai Y, Watanabe M, Ohtsuka T, Manabe T
    • Journal Title

      Eur J Neurosci.

      Volume: 44 Issue: 5 Pages: 2272-2284

    • DOI

      10.1111/ejn.13331

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Medial septal GABAergic projection neurons promote object exploration behavior and Type 2 theta rhythm.2016

    • Author(s)
      Gangadharan G, Shin J, Kim S-W, Kim A, Paydar A, Kim D-S, Miyazaki T, Watanabe M, Yanagawa Y, Kim J, Kim Y-S, Kim D, Shin H-S.
    • Journal Title

      Proc. Natl. Acad. Sci. USA,

      Volume: 113 Issue: 23 Pages: 6550-6555

    • DOI

      10.1073/pnas.1605019113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Territories of heterologous inputs onto purkinje cell dendrites are segregated by mglur1-dependent parallel fiber synapse elimination2016

    • Author(s)
      Ichikawa R, Hashimoto K, Miyazaki T, Uchigashima M, Yamasaki M, Aiba A, Kano M, Watanabe M
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 113 Issue: 8 Pages: 2282

    • DOI

      10.1073/pnas.1511513113

    • NAID

      120005828473

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] VGluT3-Expressing CCK-Positive Basket Cells Construct Invaginating Synapses Enriched with Endocannabinoid Signaling Proteins in Particular Cortical and Cortex-Like Amygdaloid Regions of Mouse Brains.2015

    • Author(s)
      Omiya Y, Uchigashima M, Konno K, Yamasaki M, Miyazaki T, Yoshida T, Kusumi I, Watanabe M.
    • Journal Title

      J Neurosci.

      Volume: 35(10) Issue: 10 Pages: 4215-4228

    • DOI

      10.1523/jneurosci.4681-14.2015

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structure–function relationships between aldolase C/Zebrin II expression and complex spike synchrony in the cerebellum2015

    • Author(s)
      Tsutsumi S, Yamazaki M, Miyazaki T, Watanabe M, Sakimura K, Kano M, Kitamura K
    • Journal Title

      J Neurosci.

      Volume: 35 Issue: 2 Pages: 843

    • DOI

      10.1523/jneurosci.2170-14.2015

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial temporal lobe epilepsy.2015

    • Author(s)
      Yokoi N, Fukata Y, Kase D, Miyazaki T, Jaegle M, Ohkawa T, Takahashi N, Iwanari H, Mochizuki Y, Hamakubo T, Imoto K, Meijer D, Watanabe M, Fukata M
    • Journal Title

      Nat Med

      Volume: 21 Issue: 1 Pages: 19-26

    • DOI

      10.1038/nm.3759

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cerebellar plasticity and motor learning deficits in a copy-number variation mouse model of autism2014

    • Author(s)
      Pichon, C., Kloth, A. D., Grasselli, G., Titley, H. K., Nakayama, H., Hashimoto, K., Wan, V., Simmons, D. H., Eissa, T., Nakatani, J., Cherskov, A., Miyazaki, T., Watanabe, M., Takumi T., Kano, M., Wang S. S-H. and Hansel, C.
    • Journal Title

      Nat. Commun.

      Volume: 5 Issue: 1 Pages: 5586-5586

    • DOI

      10.1038/ncomms6586

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A new mouse allele of glutamate receptor delta 2 with cerebellar atrophy and progressive ataxia2014

    • Author(s)
      Miyoshi, Y., Yoshioka, Y., Suzuki, K., Miyazaki, T., Koura, M., Saigoh, K., Kajimura, N., Monobe,Y., Kusunoki, S., Matsuda, J., Watanabe, M. and Hayakawa, N.
    • Journal Title

      PLoS ONE

      Volume: 9 (9) Issue: 9 Pages: 1-12

    • DOI

      10.1371/journal.pone.0107867

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Global scaling down of excitatory postsynaptic responses in cerebellar purkinje cells impairs developmental synapse elimination2014

    • Author(s)
      Kawata S, Miyazaki T, Yamazaki M, Mikuni T, Yamasaki M, Hashimoto K, Watanabe M, Sakimura K, Kano M.
    • Journal Title

      Cell Rep.

      Volume: 8 Issue: 4 Pages: 1119

    • DOI

      10.1016/j.celrep.2014.07.014

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Neuron type- and input pathway-dependent expression of Slc4a10 in adult mouse brains.2014

    • Author(s)
      Song X, Yamasaki M, Miyazaki T, Konno K, Uchigashima M, Watanabe M
    • Journal Title

      Eur J Neurosci,

      Volume: 40 Issue: 5 Pages: 2797-2810

    • DOI

      10.1111/ejn.12636

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Enriched Expression of GluD1 in Higher Brain Regions and Its Involvement in Parallel Fiber-Interneuron Synapse Formation in the Cerebellum.2014

    • Author(s)
      Konno K, Matsuda K, Nakamoto C, Uchigashima M, Miyazaki T, Yamasaki M, Sakimura K, Yuzaki M, Watanabe M.
    • Journal Title

      J Neurosci.

      Volume: 34(22) Issue: 22 Pages: 7412-24

    • DOI

      10.1523/jneurosci.0628-14.2014

    • NAID

      120005537463

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Calcineurin B1 subunit is essential for climbing fiber mono-innervation and inhibitory synapse formation in cerebellar Purkinje cells2017

    • Author(s)
      Taisuke Miyazaki, Kenji Sakimura, Masahiko Watanabe
    • Organizer
      第122回日本解剖学会総会
    • Place of Presentation
      長崎大学坂本キャンパス(長崎県長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Cav2.1 is indispensable to maintain wiring, survival, and compartmentalized expression in adult cerebellar Purkinje cells2016

    • Author(s)
      Taisuke Miyazaki, Miwako Yamasaki, Kenji Sakimura, Masahiko Watanabe
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜 (神奈川県横浜市)
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] Neuroanatomical properties of cerebellar interneuron Lugaro cells using with GFP–expressing transgenic mice line2016

    • Author(s)
      Taisuke Miyazaki, Kenji F. Tanaka, Masahiko Watanabe
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレットふくしま(福島県・郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Morphological characteristics of cerebellar interneuron Lugaro cells using with GFP–expressing transgenic mice line2015

    • Author(s)
      Taisuke Miyazaki, Kenji F. Tanaka, Masahiko Watanabe
    • Organizer
      Neuroscience 2015
    • Place of Presentation
      McCormick Place (Chicago, IL)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] プルキンエ細胞に発現するカルシニューリンCNB1サブユニットは小脳の興奮性および抑制性シナプス回路形成に重要である2015

    • Author(s)
      宮崎 太輔, 崎村 建司, 渡辺 雅彦
    • Organizer
      第61回東北・北海道連合支部学術集会
    • Place of Presentation
      盛岡市観光文化交流センター (岩手県・盛岡市)
    • Year and Date
      2015-08-29
    • Related Report
      2015 Research-status Report
  • [Presentation] プルキンエ細胞に発現するCNB1は小脳の興奮性および抑制性シナプス回路形成に重要である2015

    • Author(s)
      Taisuke Miyazaki, Kenji Sakimura , Masahiko Watanabe
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場 (兵庫県・神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Calcineurin B1 subunit expressed in Purkinje cell is essential for the formation of excitatory and inhibitory cerebellar neuronal network.2015

    • Author(s)
      Taisuke Miyazaki, Kenji Sakimura , Masahiko Watanabe
    • Organizer
      第120回日本解剖学会総会・全国学術集会
    • Place of Presentation
      神戸国際会議場(兵庫県・神戸市)
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 蛍光タンパク発現モデルマウスを用いた小脳ルガロ細胞に関する形態学解析2014

    • Author(s)
      宮崎 太輔
    • Organizer
      日本顕微鏡学会第70回記念学術講演会
    • Place of Presentation
      幕張メッセ(千葉県・幕張市)
    • Year and Date
      2014-05-11 – 2014-05-13
    • Related Report
      2014 Research-status Report
  • [Book] Immunocytochemistry and Related Techniques. Chapter 16. Combined immunocytochemistry and tracing of neural connections.2015

    • Author(s)
      Taisuke Miyazaki and Masahiko Watanabe
    • Total Pages
      14
    • Publisher
      Humana Press.
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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