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Analysis on nAChRs in the somatosensory system

Research Project

Project/Area Number 26460251
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionHokkaido University

Principal Investigator

Miwako Yamasaki  北海道大学, 医学研究科, 准教授 (10431305)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsアセチルコリン / 体性感覚野 / アセチルコリン受容体 / マウス
Outline of Final Research Achievements

In the present study, I investigated the cellular expression of nicotinic acetylcholine receptor (nAChR) subunits in the mouse somatosensory system. I made specific riboprobes for a2, a4, a7 and b2 nAChR, and found that their expression levels in the somatosensory system were below the detection threshold. Then I took another approach to localize a7 nAChR by assessing its interacting protein TMEM35 (NACHO). I raised a specific antibody and riboprobe for NACHO and found that NACHO was more highly expressed in glutamatergic neurons than GABAergic neurons in the trigeminal nuclei. On the contrary, it was much higher in GABAergic neurons than glutamatergic neurons in the VPM and somatosensory cortex. These results suggest that the expression pattern of a7 nAChR is nearly opposite in the trigeminal nuclei and its upstream regions, thereby playing distinct functions in the somatosensory system.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (22 results)

All 2017 2016 2015 2014

All Journal Article (17 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 17 results,  Open Access: 12 results,  Acknowledgement Compliant: 10 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] QRFP-deficient mice are hypophagic, lean, hypoactive and exhibit increased anxiety-like behavior.2016

    • Author(s)
      Okamoto K, Yamasaki M, Takao K, Sasaki K, Magoori K, Sakakibara I, Miyakawa T, Mieda M, Watanabe M, Sakai J, Yanagisawa M, Sakurai T:
    • Journal Title

      PLoS One

      Volume: 11

    • NAID

      120007129233

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Locus coeruleus and dopaminergic consolidation of everyday memory.2016

    • Author(s)
      Takeuchi T, Duszkiewicz AJ, Sonneborn A, Spooner PA, Yamasaki M, Watanabe M, Smith GC, Fernández G, Deisseroth K, Greene RW, Morris RGM
    • Journal Title

      Nature,

      Volume: 537 Issue: 7620 Pages: 357-362

    • DOI

      10.1038/nature19325

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus.2016

    • Author(s)
      Kobayashi S, Hida Y, Ishizaki H, Inoue E, Tanaka-Okamoto M, Yamasaki M, Miyazaki T, Masahiro F, Kitajima I, Takai Y, Watanabe M, Ohtsuka T, Manabe T
    • Journal Title

      Eur J Neurosci.

      Volume: 44 Issue: 5 Pages: 2272-2284

    • DOI

      10.1111/ejn.13331

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ionic Basis for Membrane Potential Resonance in Neurons of the Inferior Olive2016

    • Author(s)
      Matsumoto-Makidono, Y., Nakayama, H., Yamasaki, M., Miyazaki, T., Kobayashi, K., Watanabe, M., Kano, M., Sakimura, K., Hashimoto, K.
    • Journal Title

      Cell Reports

      Volume: 16 Issue: 4 Pages: 994

    • DOI

      10.1016/j.celrep.2016.06.053

    • URL

      https://pure.teikyo.jp/en/publications/3ce87e4a-7df7-4d28-8c16-070ceff28970

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Developmental switch in spike timing-dependent plasticity and cannabinoid-dependent Reorganization of the thalamocortical projection in the barrel cortex.2016

    • Author(s)
      Itami C, Huang JY, Yamasaki M, Watanabe M, Lu HC, Kimura F:
    • Journal Title

      J Neurosci.

      Volume: 36 Pages: 7039-7054

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Distinct subunit domains govern synaptic stability and specificity of the kainate receptor.2016

    • Author(s)
      Straub C, Noam Y, Nomura T, Yamasaki M, Yan D, Fernandes H, Watanabe M, Contractor A, Tomita S
    • Journal Title

      Cell Rep.

      Volume: 16 Issue: 2 Pages: 531-534

    • DOI

      10.1016/j.celrep.2016.05.093

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transsynaptic Modulation of Kainate Receptor Functions by C1q-like Proteins2016

    • Author(s)
      Matsuda K, Budisantoso T, Mitakidis N, Sugaya Y, Miura E, Kakegawa W, Yamasaki M, Konno K, Uchigashima M, Abe M, Watanabe I, Kano M, Watanabe M, Sakimura K, Aricescu AR, Yuzaki M.
    • Journal Title

      Neuron

      Volume: 90 Issue: 4 Pages: 752

    • DOI

      10.1016/j.neuron.2016.04.001

    • URL

      https://localhost/en/publications/d8d05ba5-11eb-4f5d-8f67-0eb097d2e36e

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] TARP γ-2 and γ-8 Differentially Control AMPAR Density Across Schaffer Collateral/Commissural Synapses in the Hippocampal CA1 Area.2016

    • Author(s)
      Yamasaki M, Fukaya M, Yamazaki M, Azechi H, Natsume R, Abe M, Sakimura K, Watanabe M
    • Journal Title

      J Neurosci

      Volume: 36 Issue: 15 Pages: 4296-4312

    • DOI

      10.1523/jneurosci.4178-15.2016

    • NAID

      120005849916

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Territories of heterologous inputs onto purkinje cell dendrites are segregated by mglur1-dependent parallel fiber synapse elimination2016

    • Author(s)
      Ichikawa R, Hashimoto K, Miyazaki T, Uchigashima M, Yamasaki M, Aiba A, Kano M, Watanabe M
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 113 Issue: 8 Pages: 2282

    • DOI

      10.1073/pnas.1511513113

    • NAID

      120005828473

    • URL

      https://pure.teikyo.jp/en/publications/ef6116f6-3235-46cc-b619-656ef2bb4818

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular and anatomical evidence for the input pathway- and target cell type-dependent regulation of glutamatergic synapses.2016

    • Author(s)
      Yamasaki M
    • Journal Title

      Anat Sci Int

      Volume: 91 Issue: 1 Pages: 8-21

    • DOI

      10.1007/s12565-015-0303-0

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] VGluT3-Expressing CCK-Positive Basket Cells Construct Invaginating Synapses Enriched with Endocannabinoid Signaling Proteins in Particular Cortical and Cortex-Like Amygdaloid Regions of Mouse Brains.2015

    • Author(s)
      Omiya Y, Uchigashima M, Konno K, Yamasaki M, Miyazaki T, Yoshida T, Kusumi I, Watanabe M.
    • Journal Title

      J Neurosci.

      Volume: 35(10) Issue: 10 Pages: 4215-4228

    • DOI

      10.1523/jneurosci.4681-14.2015

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Involvement of diacylglycerol kinase B in the spine formation at distal dendrites of striatal medium spiny neurons.2015

    • Author(s)
      Hozumi Y, Kakefuda K, Yamasaki M, Watanabe M, Hara H, Goto K
    • Journal Title

      Brain Res,

      Volume: 1594 Pages: 36-45

    • DOI

      10.1016/j.brainres.2014.11.012

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] The glutamate receptor GluN2 subunit regulates synaptic trafficking of AMPA receptors in the neonatal mouse brain2014

    • Author(s)
      Hamada, S., Ogawa, I., Yamasaki, M., Kiyama, Y., Watabe, A. M., Kassai, H., Nakano, K., Aiba, A., Watanabe, M. and Manabe, T.
    • Journal Title

      European Journal of Neuroscience

      Volume: 40 Issue: 8 Pages: 3136-3146

    • DOI

      10.1111/ejn.12682

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Global scaling down of excitatory postsynaptic responses in cerebellar purkinje cells impairs developmental synapse elimination2014

    • Author(s)
      Kawata S, Miyazaki T, Yamazaki M, Mikuni T, Yamasaki M, Hashimoto K, Watanabe M, Sakimura K, Kano M.
    • Journal Title

      Cell Rep.

      Volume: 8 Issue: 4 Pages: 1119

    • DOI

      10.1016/j.celrep.2014.07.014

    • URL

      https://pure.teikyo.jp/en/publications/2079c7db-ebb4-499b-af8c-505bb234affb

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Opposing role of NMDA receptor GluN2B and GluN2D in somatosensory development and maturation2014

    • Author(s)
      Miwako Yamasaki, Rieko Okada, Chihiro Takasaki, Shima Toki, Rie Natsume, Kenji Sakimura, Masayoshi Mishina, Tetsuo Shirakawa and Masahiko Watanabe
    • Journal Title

      The Journal of Neuroscience

      Volume: 34 Issue: 35 Pages: 11534-11548

    • DOI

      10.1523/jneurosci.1811-14.2014

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Neuron type- and input pathway-dependent expression of Slc4a10 in adult mouse brains.2014

    • Author(s)
      Song X, Yamasaki M, Miyazaki T, Konno K, Uchigashima M, Watanabe M
    • Journal Title

      Eur J Neurosci,

      Volume: 40 Issue: 5 Pages: 2797-2810

    • DOI

      10.1111/ejn.12636

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Enriched Expression of GluD1 in Higher Brain Regions and Its Involvement in Parallel Fiber-Interneuron Synapse Formation in the Cerebellum.2014

    • Author(s)
      Konno K, Matsuda K, Nakamoto C, Uchigashima M, Miyazaki T, Yamasaki M, Sakimura K, Yuzaki M, Watanabe M.
    • Journal Title

      J Neurosci.

      Volume: 34(22) Issue: 22 Pages: 7412-24

    • DOI

      10.1523/jneurosci.0628-14.2014

    • NAID

      120005537463

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] マウス線条体シナプスのAMPA受容体密度は細胞種や出入力経路によらず一様である2017

    • Author(s)
      山崎美和子、宋暁紅、渡辺雅彦
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎大学(長崎県、長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 小脳登上線維と抑制性細胞間における非シナプス性結合と選択的分子発現2016

    • Author(s)
      山崎美和子、深谷昌弘、宋暁紅、内ヶ島基政、宮崎太輔、今野幸太郎、渡辺雅彦
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2016-03-29
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Molecular determinants underlying heterogeneity in AMPAR content across CA1 Schaffer collateral synapses.2015

    • Author(s)
      Yamasaki M, Fukaya M, Yamazaki M, Azechi H, Natsume R, Abe M, Sakimura K, Watanabe M
    • Organizer
      Neuroscience 2015 (45th Society for neuroscience annual meeting)
    • Place of Presentation
      McCormick Place (Chicago, USA)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Opposing role of NMDA receptor GluN2B and GluN2D in somatosensory development and maturation2015

    • Author(s)
      1.Yamasaki M, Okada R, Takasaki C, Toki S, Fukaya M, Natsume R, Sakimura K, Mishina M, Shirakawa T, Watanabe M
    • Organizer
      第120回日本解剖学会総会・全国学術集会
    • Place of Presentation
      神戸国際会議場(兵庫県・神戸市)
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Book] Receptor and Ion Channel Detection in the Brain2016

    • Author(s)
      Yamasaki M, Watanabe M
    • Total Pages
      475
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report 2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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