Analysis on nAChRs in the somatosensory system

• Project/Area Number: 26460251
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Hokkaido University
• Principal Investigator: Miwako Yamasaki 北海道大学, 医学研究科, 准教授 (10431305)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アセチルコリン / 体性感覚野 / アセチルコリン受容体 / マウス

Outline of Final Research Achievements

In the present study, I investigated the cellular expression of nicotinic acetylcholine receptor (nAChR) subunits in the mouse somatosensory system. I made specific riboprobes for a2, a4, a7 and b2 nAChR, and found that their expression levels in the somatosensory system were below the detection threshold. Then I took another approach to localize a7 nAChR by assessing its interacting protein TMEM35 (NACHO). I raised a specific antibody and riboprobe for NACHO and found that NACHO was more highly expressed in glutamatergic neurons than GABAergic neurons in the trigeminal nuclei. On the contrary, it was much higher in GABAergic neurons than glutamatergic neurons in the VPM and somatosensory cortex. These results suggest that the expression pattern of a7 nAChR is nearly opposite in the trigeminal nuclei and its upstream regions, thereby playing distinct functions in the somatosensory system.

Research Products

• [Journal Article] QRFP-deficient mice are hypophagic, lean, hypoactive and exhibit increased anxiety-like behavior. (2016)
  • Author(s): Okamoto K, Yamasaki M, Takao K, Sasaki K, Magoori K, Sakakibara I, Miyakawa T, Mieda M, Watanabe M, Sakai J, Yanagisawa M, Sakurai T:
  • Journal Title: PLoS One Volume: 11
  • NAID: 120007129233
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Locus coeruleus and dopaminergic consolidation of everyday memory. (2016)
  • Author(s): Takeuchi T, Duszkiewicz AJ, Sonneborn A, Spooner PA, Yamasaki M, Watanabe M, Smith GC, Fernández G, Deisseroth K, Greene RW, Morris RGM
  • Journal Title: Nature, Volume: 537 Issue: 7620 Pages: 357-362
  • DOI: 10.1038/nature19325
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus. (2016)
  • Author(s): Kobayashi S, Hida Y, Ishizaki H, Inoue E, Tanaka-Okamoto M, Yamasaki M, Miyazaki T, Masahiro F, Kitajima I, Takai Y, Watanabe M, Ohtsuka T, Manabe T
  • Journal Title: Eur J Neurosci. Volume: 44 Issue: 5 Pages: 2272-2284
  • DOI: 10.1111/ejn.13331
  • Peer Reviewed / Open Access
• [Journal Article] Ionic Basis for Membrane Potential Resonance in Neurons of the Inferior Olive. (2016)
  • Author(s): Matsumoto-Makidono, Y., Nakayama, H., Yamasaki, M., Miyazaki, T., Kobayashi, K., Watanabe, M., Kano, M., Sakimura, K., Hashimoto, K.
  • Journal Title: Cell Reports Volume: 16 Issue: 4 Pages: 994-1004
  • DOI: 10.1016/j.celrep.2016.06.053
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Developmental switch in spike timing-dependent plasticity and cannabinoid-dependent Reorganization of the thalamocortical projection in the barrel cortex. (2016)
  • Author(s): Itami C, Huang JY, Yamasaki M, Watanabe M, Lu HC, Kimura F:
  • Journal Title: J Neurosci. Volume: 36 Pages: 7039-7054
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Distinct subunit domains govern synaptic stability and specificity of the kainate receptor. (2016)
  • Author(s): Straub C, Noam Y, Nomura T, Yamasaki M, Yan D, Fernandes H, Watanabe M, Contractor A, Tomita S
  • Journal Title: Cell Rep. Volume: 16 Issue: 2 Pages: 531-534
  • DOI: 10.1016/j.celrep.2016.05.093
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Trans-synaptic modulation of kainate receptor functions by C1q-like proteins. (2016)
  • Author(s): Matsuda K, Budisantoso T, Mitakidis N, Sugaya Y, Miura E, Kakegawa W, Yamasaki M, Konno K, Uchigashima M, Abe M, Watanabe I, Kano M, Watanabe M, Sakimura K, Aricescu AR, Yuzaki M.
  • Journal Title: Neuron Volume: in press Issue: 4 Pages: 752-767
  • DOI: 10.1016/j.neuron.2016.04.001
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] TARP γ-2 and γ-8 Differentially Control AMPAR Density Across Schaffer Collateral/Commissural Synapses in the Hippocampal CA1 Area. (2016)
  • Author(s): Yamasaki M, Fukaya M, Yamazaki M, Azechi H, Natsume R, Abe M, Sakimura K, Watanabe M
  • Journal Title: J Neurosci Volume: 36 Issue: 15 Pages: 4296-4312
  • DOI: 10.1523/jneurosci.4178-15.2016
  • NAID: 120005849916
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Territories of heterologous inputs onto Purkinje cell dendrites are segregated by mGluR1-dependent parallel fiber synapse elimination (2016)
  • Author(s): Ichikawa R, Hashimoto K, Miyazaki T, Uchigashima M, Yamasaki M, Aiba A, Kano M, Watanabe M
  • Journal Title: Proc Natl Acad Sci U S A Volume: 113 (8) Issue: 8 Pages: 2282-7
  • DOI: 10.1073/pnas.1511513113
  • NAID: 120005828473
  • Peer Reviewed / Open Access
• [Journal Article] Molecular and anatomical evidence for the input pathway- and target cell type-dependent regulation of glutamatergic synapses. (2016)
  • Author(s): Yamasaki M
  • Journal Title: Anat Sci Int Volume: 91 Issue: 1 Pages: 8-21
  • DOI: 10.1007/s12565-015-0303-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] VGluT3-Expressing CCK-Positive Basket Cells Construct Invaginating Synapses Enriched with Endocannabinoid Signaling Proteins in Particular Cortical and Cortex-Like Amygdaloid Regions of Mouse Brains. (2015)
  • Author(s): Omiya Y, Uchigashima M, Konno K, Yamasaki M, Miyazaki T, Yoshida T, Kusumi I, Watanabe M.
  • Journal Title: J Neurosci. Volume: 35(10) Issue: 10 Pages: 4215-4228
  • DOI: 10.1523/jneurosci.4681-14.2015
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Involvement of diacylglycerol kinase B in the spine formation at distal dendrites of striatal medium spiny neurons. (2015)
  • Author(s): Hozumi Y, Kakefuda K, Yamasaki M, Watanabe M, Hara H, Goto K
  • Journal Title: Brain Res, Volume: 1594 Pages: 36-45
  • DOI: 10.1016/j.brainres.2014.11.012
  • Peer Reviewed
• [Journal Article] The glutamate receptor GluN2 subunit regulates synaptic trafficking of AMPA receptors in the neonatal mouse brain (2014)
  • Author(s): Hamada, S., Ogawa, I., Yamasaki, M., Kiyama, Y., Watabe, A. M., Kassai, H., Nakano, K., Aiba, A., Watanabe, M. and Manabe, T.
  • Journal Title: European Journal of Neuroscience Volume: 40 Issue: 8 Pages: 3136-3146
  • DOI: 10.1111/ejn.12682
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Global Scaling Down of Excitatory Postsynaptic Responses in Cerebellar Purkinje Cells Impairs Developmental Synapse Elimination. (2014)
  • Author(s): Kawata S, Miyazaki T, Yamazaki M, Mikuni T, Yamasaki M, Hashimoto K, Watanabe M, Sakimura K, Kano M.
  • Journal Title: Cell Rep. Volume: 8(4) Issue: 4 Pages: 1119-29
  • DOI: 10.1016/j.celrep.2014.07.014
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Opposing role of NMDA receptor GluN2B and GluN2D in somatosensory development and maturation (2014)
  • Author(s): Miwako Yamasaki, Rieko Okada, Chihiro Takasaki, Shima Toki, Rie Natsume, Kenji Sakimura, Masayoshi Mishina, Tetsuo Shirakawa and Masahiko Watanabe
  • Journal Title: The Journal of Neuroscience Volume: 34 Issue: 35 Pages: 11534-11548
  • DOI: 10.1523/jneurosci.1811-14.2014
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Neuron type- and input pathway-dependent expression of Slc4a10 in adult mouse brains. (2014)
  • Author(s): Song X, Yamasaki M, Miyazaki T, Konno K, Uchigashima M, Watanabe M
  • Journal Title: Eur J Neurosci, Volume: 40 Issue: 5 Pages: 2797-2810
  • DOI: 10.1111/ejn.12636
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Enriched Expression of GluD1 in Higher Brain Regions and Its Involvement in Parallel Fiber-Interneuron Synapse Formation in the Cerebellum. (2014)
  • Author(s): Konno K, Matsuda K, Nakamoto C, Uchigashima M, Miyazaki T, Yamasaki M, Sakimura K, Yuzaki M, Watanabe M.
  • Journal Title: J Neurosci. Volume: 34(22) Issue: 22 Pages: 7412-24
  • DOI: 10.1523/jneurosci.0628-14.2014
  • NAID: 120005537463
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] マウス線条体シナプスのAMPA受容体密度は細胞種や出入力経路によらず一様である (2017)
  • Author(s): 山崎美和子、宋暁紅、渡辺雅彦
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学（長崎県、長崎市）
  • Year and Date: 2017-03-28
• [Presentation] 小脳登上線維と抑制性細胞間における非シナプス性結合と選択的分子発現 (2016)
  • Author(s): 山崎美和子、深谷昌弘、宋暁紅、内ヶ島基政、宮崎太輔、今野幸太郎、渡辺雅彦
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビッグパレットふくしま(福島県郡山市）
  • Year and Date: 2016-03-29
  • Invited
• [Presentation] Molecular determinants underlying heterogeneity in AMPAR content across CA1 Schaffer collateral synapses. (2015)
  • Author(s): Yamasaki M, Fukaya M, Yamazaki M, Azechi H, Natsume R, Abe M, Sakimura K, Watanabe M
  • Organizer: Neuroscience 2015 (45th Society for neuroscience annual meeting)
  • Place of Presentation: McCormick Place (Chicago, USA)
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Opposing role of NMDA receptor GluN2B and GluN2D in somatosensory development and maturation (2015)
  • Author(s): 1.Yamasaki M, Okada R, Takasaki C, Toki S, Fukaya M, Natsume R, Sakimura K, Mishina M, Shirakawa T, Watanabe M
  • Organizer: 第１２０回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Book] Receptor and Ion Channel Detection in the Brain (2016)
  • Author(s): Yamasaki M, Watanabe M
  • Total Pages: 475
  • Publisher: Springer