Developmental modularities behind evolution of the jaw and middle ear in mammals

• Project/Area Number: 26460256
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: TAKECHI Masaki 東京医科歯科大学, 大学院医歯学総合研究科, テニュアトラック助教 (10455355)
• Research Collaborator: FURUTERA Toshiko
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 中耳 / 鼓膜 / 外耳道 / 咽頭弓 / 進化発生 / マウス / ニワトリ / 下顎 / 形態進化 / 発生進化 / Hoxa2 / Otx2

Outline of Final Research Achievements

We have focused on morphological evolution of the mammalian middle ear and have reported that the tympanic membrane (TM), consisting of the external auditory meatus (EAM) and first pharyngeal pouch, evolved independently in mammalian and diapsid (modern reptiles and birds) lineages. In this study, we compare TM formation along the anterior-posterior axis of pharyngeal arches in mouse and chick embryos. In mouse, TM is formed in the first pharyngeal arch (PA1) and this arch plays a crucial role in TM formation. By contrast, the EAM begins to invaginate at the surface ectoderm of the second pharyngeal arch (PA2) in chick, and the entire TM is formed in PA2. These results indicate that the classical idea of TM formation is not correct in either animal and further strengthen the idea of independent origin of the TM during amniote evolution.

Research Products

• [Int'l Joint Research] Friedrich Miescher Institute(スイス連邦)
• [Journal Article] Differing contributions of the first and second pharyngeal arches to tympanic membrane formation in the mouse and chick (2017)
  • Author(s): Furutera T, Takechi M, Kitazawa T, Takei J, Yamada T, Vu Hoang T, Rijli FM, Kurihara H, Kuratani S, Iseki S.
  • Journal Title: Development Volume: 144 Pages: 3315-3324
  • DOI: 10.1242/dev.149765
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Developmental mechanisms of the tympanic membrane in mammals and non-mammalian amniotes (2016)
  • Author(s): Takechi, M., Kitazawa, T., Hirasawa, T., Hirai, T., Iseki, S., Kurihara, H., Kuratani, S.
  • Journal Title: Congenital Anomalies Volume: 56 Issue: 1 Pages: 12-17
  • DOI: 10.1111/cga.12132
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 進化発生学から探る哺乳類の中耳の進化 (2015)
  • Author(s): 武智正樹、北沢太郎、栗原裕基、倉谷滋
  • Journal Title: 生体の科学 Volume: 66 Pages: 234-239
• [Presentation] Differing contributions of the first and second pharyngeal arches to tympanic membrane formation in the mouse and chick. (2018)
  • Author(s): Takechi. M., Furutera, T., Kitazawa, T., Rijli, F., Kurihara. H., Kuratani, S., Iseki, S.
  • Organizer: Gordon Research Conference, Craniofacial Morphogenesis and Tissue Regeneration
  • Int'l Joint Research
• [Presentation] 鼓膜は本当に第１咽頭弓と第２咽頭弓の境界に形成されるのか？ (2018)
  • Author(s): 武智正樹
  • Organizer: 第123回日本解剖学会総会・全国学術集会
  • Invited
• [Presentation] 哺乳類中耳の形態進化における分子発生学的背景について (2017)
  • Author(s): 武智正樹
  • Organizer: 第122回日本解剖学会総会・全国学術集会シンポジウム
  • Place of Presentation: 長崎大学（長崎県長崎市）
  • Year and Date: 2017-03-30
  • Invited
• [Presentation] Differing contributions of the first and second pharyngeal arches to tympanic membrane formation in the mouse and chick (2017)
  • Author(s): Takechi, M., Furutera, T., Kitazawa, T., Takei, J., Yamada, T., Rijli, F. M., Kurihara, H., Kuratani, S., Iseki. S.
  • Organizer: 50th Annual meeting for Japanese Society of Developmental Biologists
• [Presentation] 神経堤細胞から形成される顎と中耳の進化発生学 (2016)
  • Author(s): 武智正樹
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-12-02
  • Invited
• [Presentation] 哺乳類と爬虫類‐鳥類の鼓膜形成メカニズムはどのように異なるのか？ (2016)
  • Author(s): 古寺敏子、武智正樹、北沢太郎、栗原裕基、倉谷滋、井関祥子
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-12-01
• [Presentation] 中耳の形態進化を発生学的に理解する (2016)
  • Author(s): 武智正樹
  • Organizer: 日本進化学会第18回大会
  • Place of Presentation: 東京工業大学（東京都大田区）
  • Year and Date: 2016-08-26
  • Invited
• [Presentation] 鼓膜形成の比較発生学的解析 (2016)
  • Author(s): 武智正樹、古寺敏子、顧潔、横山紀典、井関祥子
  • Organizer: 第6回 Tokyo Vertebrate Morphology Meeting
  • Place of Presentation: 東京慈恵会医科大学（東京都港区）
  • Year and Date: 2016-08-06
• [Presentation] 鼓膜の発生メカニズムとその進化的考察 (2016)
  • Author(s): 武智正樹、顧潔、横山紀典、古寺敏子、井関祥子
  • Organizer: 第56回日本先天異常学会学術集会
  • Place of Presentation: 姫路商工会議所（兵庫県姫路市）
  • Year and Date: 2016-07-29
• [Presentation] 顎と中耳の発生における背腹軸・前後軸に沿ったパターニング機構の解析 (2016)
  • Author(s): 武智正樹、北沢太郎、平井珠美、横山紀典、栗原由紀子、栗原裕基、倉谷滋、井関祥子
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 郡山ビッグパレット（福島県郡山市）
  • Year and Date: 2016-03-28
• [Presentation] 哺乳類と双弓類は異なる発生機構によって独立に鼓膜を獲得した (2015)
  • Author(s): 武智正樹、北沢太郎、栗原由紀子、井関祥子、栗原裕基、倉谷滋
  • Organizer: 日本進化学会第17回大会
  • Place of Presentation: 中央大学（東京都文京区）
  • Year and Date: 2015-08-20
• [Presentation] なぜ哺乳類のみが３つの耳小骨をもつのか？ (2015)
  • Author(s): 武智正樹、北沢太郎、井関祥子、栗原由紀子、栗原裕基、倉谷滋
  • Organizer: 第55回日本先天異常学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2015-07-25
• [Remarks] 鼓膜の発生過程が従来の理解と異なることを発見
  • URL: http://www.tmd.ac.jp/archive-tmdu/kouhou/20170919_2.pdf
• [Remarks]
  • URL: http://reins.tmd.ac.jp/html/100009250_ja.html