| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
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| Outline of Final Research Achievements |
Pharyngeal arch (PA) is a transient segmental structure, and it is important for the later peripheral neurogenesis. Ripply3 is highly expressed in the developing PA of mouse. To explore the regulatory mechanism underlying between PA formation and glossopharyngeal nerve (GN) development, we focused on the involvement of Ripply3. Consequently, we found Ripply3 is required for GN development. Next, we identified retinoic acid (RA) is required for the enhancement of Ripply3 expression. Ripply3 promoter analysis revealed that RA and Ripply3 cooperatively participate in PA and GN development. We also found that Ripply3 expressing cells give rise to GN ganglia by using cre reporter system. Some candidates of Ripply3 interacting genes were identified. Among them, Paxilin is involved in morphogenesis of PA with Ripply3, and Glyr1 is a novel factor regulates the transcriptional property of Ripply3. Thus, we gained new insights into the Ripply3 function in GN formation during PA development.
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