Analysis of the neural network formation in NZF-2/-3 double knock-out mice that exhibit symptoms of arthrogryposis
| Project/Area Number |
26460259
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
亀山 俊樹 藤田医科大学, 総合医科学研究所, 助教 (60298544)
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| Project Period (FY) |
2014-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | neural zinc finger / myelin / transcription factor / 神経発生 / 神経回路 / 関節拘縮 / 遺伝子破壊マウス / トランスクリプトーム / NZF / Myt / ミエリン / 変異マウス / 転写因子 |
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| Outline of Final Research Achievements |
We generated the double knockout mice for gene silencing of NZF-2 and NZF-3, and compared these mice with normal or single knockout mice of NZF-2 or NZF-3 by their histology and gene-expression levels.
As a result, the neurons did not innervate from spinal cord into the diaphragm or the distal portion of the extremities. In cerebral cortex, the bundles of nerve fibers were completely lost. In the genes that have moderately changed expression levels in the double knockout mice compared to wild type mice, we found ones that control alternative splicing. This finding suggests that NZF-2 and NZF-3 may influence alternative splicing patterns of other genes.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で得られた結果は、神経細胞が神経線維を伸ばして情報網をつくる際に、２つの遺伝子NZF-2とNZF-3が協力して必要不可欠な働きをすることを示しています。また、正常マウスと比べて二重変異マウスでは、他の遺伝子の働き方が変化する可能性があります。こうした遺伝子やNZF-2とNZF-3の働きは、再生医療で神経を再生させたり、遺伝子の働き方の変化を検査して神経難病を診断するなどの応用が期待できます。
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Report
(7 results)
Research Products
(41 results)
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[Journal Article] Rearrangement of VPS13B, a causative gene of Cohen syndrome, in a case of RUNX1-RUNX1T1 leukemia with t(8;12;21).2017
Author(s)
Abe A, Yamamoto Y, Katsumi A, Okamoto A, Tokuda M, Inaguma Y, Yamamoto K, Yanada M, Kanie T, Tomita A, Akatsuka Y, Okamoto M, Kameyama T, Mayeda A, Emi N.
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Journal Title
Int J Hematol.
Volume: -
Issue: 2
Pages: 208-212
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] ETV6-LPXN fusion transcript generated by t(11;12)(q12.1;p13) in a patient with relapsing acute myeloid leukemia with NUP98-HOXA9.2016
Author(s)
Abe A, Yamamoto Y, Iba S, Kanie T, Okamoto A, Tokuda M, Inaguma Y, Yanada M, Morishima S, Mizuta S, Akatsuka Y, Okamoto M, Kameyama T, Mayeda A, Emi N.
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Journal Title
Genes, chromosomes & cancer
Volume: 55(3)
Issue: 3
Pages: 242-250
DOI
Related Report
Peer Reviewed
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[Journal Article] NUP214-RAC1 and RAC1-COL12A1 Fusion in Complex Variant Translocations Involving Chromosomes 6, 7 and 9 in an Acute Myeloid Leukemia Case with DEK-NUP214.2015
Author(s)
Abe A, Yamamoto Y, Iba S, Okamoto A, Tokuda M, Inaguma Y, Yanada M, Morishima S, Kanie T, Tsuzuki M, Akatsuka Y, Mizuta S, Okamoto M, Kameyama T, Mayeda A, Emi N.
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Journal Title
Cytogenetic and genome research
Volume: 146(4)
Issue: 4
Pages: 279-284
DOI
Related Report
Peer Reviewed
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[Journal Article] Fluvoxamine moderates reduced voluntary activity following chronic dexamethasone infusion in mice via2014
Author(s)
Terada K, Izumo N, Suzuki B, Karube Y, Morikawa T, Ishibashi Y, Kameyama T, Chiba K, Sasaki N, Iwata K,
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Journal Title
Neurochemistry International
Volume: 69
Pages: 9-13
DOI
Related Report
Peer Reviewed
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[Presentation] Regulation of VWF-GPIB interaction with modified recombinant botrocetin2015
Author(s)
Matsui T, Hori A, Hamako J, Matsushita F, Takagishi N, Kondo K, Kano T, Hayakawa M, Matsumoto M and Fujimura Y
Organizer
The International Society on Thrombosis and Haemostasis 2015 Congress and 61st Annual SSC Meeting
Place of Presentation
Metro Toronto Convention Center (MTCC), Toronto, Canada
Year and Date
2015-06-20
Related Report
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