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Engineering of artificial three-dimensional tissue with functional lymphatic network for regenerative medicine

Research Project

Project/Area Number 26460264
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionHirosaki University

Principal Investigator

Asano Yoshiya  弘前大学, 医学研究科, 助教 (50359494)

Co-Investigator(Kenkyū-buntansha) 下田 浩  弘前大学, 医学研究科, 教授 (20274748)
Co-Investigator(Renkei-kenkyūsha) OKANO Daisuke  弘前大学, 医学研究科, 助手 (00719023)
AKASHI Mituru  大阪大学, 工学研究科, 教授 (20145460)
MATSUSAKI Michiya  大阪大学, 工学研究科, 助教 (00419467)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords三次元人工脈管組織 / リンパ管 / 再生医療 / 人工リンパ管網組織移植 / 脈管リモデリング / 三次元積層培養技術 / 人工脈管ネットワーク / リンパ浮腫
Outline of Final Research Achievements

An artificial human vascular tissue constructed by a cell accumulation method capable of constructing a three-dimensional artificial tissue can be transplanted and expected to be applied to regenerative medicine. In this study, we aimed at transplantation of artificial human lymphatic network tissue (AHLT), fabricated by using human fibroblasts and lymphatic endothelial cells, into nude mice, and verification of its therapeutic effects. As the results, AHLT engrafted in mouse subcutaneous and fascial tissue, and artificial lymphatic vessels had the structures of lymphatic capillaries. In addition, engraftment of small blood vessel-like structures positive for human markers was also observed, and a vascular remodeling including lymphatic vessel-to-vascular conversion was suggested. Furthermore, AHLT transplantation into the lymph node-dissected model gave a result suggesting induction of lymphatic flow regeneration.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results)

  • [Journal Article] 細胞集積法によるヒト人工三次元脈管組織のエンジニアリングと移植2016

    • Author(s)
      浅野義哉, 松崎典弥, 岡野大輔, 西口昭広, 明石満, 下田 浩
    • Journal Title

      リンパ学

      Volume: 39 Pages: 18-20

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Transplantation of three-dimensional artificial human vascular tissues fabricated using an extracellular matrix nanofilm-based cell-accumulation technique2015

    • Author(s)
      Asano Y, Shimoda H, Okano D, Matsusaki M, Akashi M
    • Journal Title

      J Tissue Eng Regen Med

      Volume: in press Issue: 4 Pages: 1303-1307

    • DOI

      10.1002/term.2108

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 血管・リンパ管ネットワークを有する三次元腹膜組織2015

    • Author(s)
      浅野義哉、松崎典弥、明石満、下田浩
    • Journal Title

      化学工業

      Volume: 66 Pages: 824-830

    • Related Report
      2015 Research-status Report
  • [Presentation] 三次元人工ヒトリンパ管網組織の移植と脈管リモデリング2017

    • Author(s)
      浅野義哉、下田浩、岡野大輔、松崎典弥、明石満
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎大学坂本キャンパス (長崎県、長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 三次元人工ヒトリンパ管網組織の移植と生着後の組織形成2017

    • Author(s)
      浅野 義哉、松﨑 典弥、明石 満、下田 浩
    • Organizer
      日本再生医療学会
    • Place of Presentation
      仙台国際センター (宮城県、仙台市)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] 3次元人工腹膜組織を用いた癌腹膜転移モデル2016

    • Author(s)
      浅野義哉, 小田切 理, 永谷春香, 松崎典弥, 明石 満, 下田 浩
    • Organizer
      第121回日本解剖学会全国学術集会
    • Place of Presentation
      ビッグパレットふくしま (福島県郡山市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 細胞集積法によるヒト人工三次元脈管組織のエンジニアリングと移植2015

    • Author(s)
      浅野義哉 松﨑典弥 岡野大輔 西口昭広 明石満 下田浩
    • Organizer
      第39回日本リンパ学会総会
    • Place of Presentation
      ソラシティーカンファレンスセンター(東京)
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞集積法によって作製した血管およびリンパ管を有する人工三次元組織のエンジニアリング2015

    • Author(s)
      浅野義哉、松﨑典弥、西口昭広、岡野大輔、明石満、下田浩
    • Organizer
      第120回日本解剖学会総会・全国学術集会 シンポジウム: リンパ管の機能・形態研究の新たな展望
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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