influence of trisomy 21 on early neural differentiation

• Project/Area Number: 26460275
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Tottori University
• Principal Investigator: Hiratsuka Masaharu 鳥取大学, 医学部, 助教 (00362872)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 遺伝子発現モニター / 人工染色体 / 神経分化誘導

Outline of Final Research Achievements

Chromosomal abnormalities, such as Trisomy for human chromosome 21 (Down syndrome), are thought to be the most common cause of mental retardation. To gain insight into the underlying cellular pathogenesis, we made a new system to monitor differentiation process from forebrain neural precursor cells into interneurons and oligodendrocytes by using a human artificial chromosome vector.

Research Products

• [Journal Article] Development of a Safeguard System using an Episomal Mammalian Artificial Chromosome for Gene and Cell Therapy. (2015)
  • Author(s): Uno N, Uno K, Komoto S, Suzuki T, Hiratsuka M, Osaki M, Kazuki Y, Oshimura M.
  • Journal Title: Mol Ther Nucleic Acids. Volume: 4 Pages: e272-e272
  • DOI: 10.1038/mtna.2015.45
  • Peer Reviewed / Open Access
• [Journal Article] Retargeting of microcell fusion towards recipient cell-oriented transfer of human artificial chromosome (2015)
  • Author(s): Hiratsuka M, Ueda K, Uno N, Uno K, Fukuhara S, Kurosaki H, Takehara S, Osaki M, Kazuki Y, Kurosawa Y, Nakamura T, Katoh M, Oshimura M
  • Journal Title: BMC Biotechnol. Volume: 15 Issue: 1 Pages: 58-58
  • DOI: 10.1186/s12896-015-0142-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Remarks] 鳥取大学 医学部 生命科学科 細胞工学
  • URL: http://saiboukougaku.jimdo.com/