| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
This research project aimed to establish techniques for visualizing fine structures of cultured neurons employing a new type of electron microscope called atmospheric scanning electron microscope (ASEM). A specimen for ASEM must be set in the specialized sample dish (ASEM dish) equipped with an electron-permeable film window in its base. In this study, cerebral cortical neurons prepared from mouse embryos were cultured on the ASEM dish coated with poly-D-lysine, and fixed cells were stained with phosphotungstic acid. We thus successfully visualized filopodia in the cortical neurons with ASEM. We then utilized this technique to analyze the sprouting of filopodial protrusions on the shafts of cortical axons in response to an axon guidance cue netrin-1.
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