Analysis of structure and function of proteins involved in membrane fusion using two-photon fluorescence lifetime imaging
Project/Area Number |
26460290
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General physiology
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Research Institution | The University of Tokyo |
Principal Investigator |
Takahashi Noriko 東京大学, 大学院医学系研究科(医学部), 講師 (60332178)
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Research Collaborator |
WATANABE Satoshi
NOGUCHI Jun
SAWADA Wakako
HASAN Ucar
KASAI Haruo
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 神経伝達 / 蛋白質 / 蛍光 / 2光子励起 / 開口放出 / 膜融合 / 神経 / シナプス / 伝達 / インスリン / 蛍光色素 / 内分泌 / 分泌 / シナプス伝達 / 蛍光寿命 / イメージング |
Outline of Final Research Achievements |
Membrane fusion is involved in neurotransmitter release and hormone secretion. Using two-photon fluorescence lifetime imaging and fluorescence labeling techniques of proteins, we measured assembly of proteins involved in membrane fusion, and detected the conformational change during the exocytosis. We detected the significant assembly of SNARE molecules in the presynaptic terminals, and oligomerization with the linker of SNAP25 was required for the ultrafast exocytosis. Moreover, such assembly was considered to be related with release probability. However, in the resting conditions in pancreatic beta cells, seldom assebly was detected, indicating that stimulants those increase cytosolic calciumconcentration is neccesary for assembling SNARE molecules.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Munc18b increases insulin granule fusion, restoring deficient insulin secretion in type-2 diabetes human and Goto-Kakizaki rat islets with improvement in glucose homeostasis.2017
Author(s)
Qin T, Liang T, Zhu D, Kang Y, Xie L, Dolai S, Sugita S, Takahashi N, Ostenson CG, Banks K, Gaisano HY
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Journal Title
EBioMedicine
Volume: 16
Pages: 262-274
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Design and synthesis of a new chromophore, 2-(4-nitrophenyl)benzofuran, for two-photon uncaging using near-IR light2016
Author(s)
Komori, N.; Jakkampudi, S.; Motoishi, R.; Abe, M.; Kamada, K.; Furukawa, K.; Katan, C.; Sawada, W.; Takahashi, N.; Kasai, H.; Xue, B.; Kobayashi, T.
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Journal Title
Chemical Communications
Volume: 52
Issue: 2
Pages: 331-334
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] State-dependent diffusion of actin-depolymerizing factor/cofilin underlies the enlargement and shrinkage of dendritic spines.2016
Author(s)
Noguchi, J., Hayama, T., Watanabe, S., Ucar, H., Yagishita, S., Takahashi, N. & Kasai, H.
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 32897-32897
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] 膵内分泌腺の開口放出機構2016
Author(s)
高橋倫子、澤田和可子、野口潤、河西春郎
Organizer
第121回日本解剖学会総会・全国学術集会
Place of Presentation
ビッグパレットふくしま(福島県郡山市)
Year and Date
2016-03-28
Related Report
Invited
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