| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Membrane fusion is involved in neurotransmitter release and hormone secretion. Using two-photon fluorescence lifetime imaging and fluorescence labeling techniques of proteins, we measured assembly of proteins involved in membrane fusion, and detected the conformational change during the exocytosis. We detected the significant assembly of SNARE molecules in the presynaptic terminals, and oligomerization with the linker of SNAP25 was required for the ultrafast exocytosis. Moreover, such assembly was considered to be related with release probability. However, in the resting conditions in pancreatic beta cells, seldom assebly was detected, indicating that stimulants those increase cytosolic calciumconcentration is neccesary for assembling SNARE molecules.
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