| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Cardiac glycosides, specific Na,K-ATPase inhibitors, has been suggested to be a valuable anti-cancer drugs. However, the molecular mechanisms for anti-cancer effects of the drugs has not been fully understood. In this study, we have clarified that non-pumping (receptor type) Na,K-ATPase is functionally coupled with volume-sensitive outwardly rectifying (VSOR) anion channels in membrane microdomains of human cancer cells. Submicromolar ouabain activates the cancer cells-specific crosstalk between Na,K-ATPase and VSOR channels in membrane microdomains mediated by NADPH oxidase-dependent reactive oxygen species generation. The activation of VSOR channels elicits anti-proliferative effects in cancer cells.
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