Regulation of membrane trafficking through PI(3)P-metabolizing enzymes in endothelial cells.
| Project/Area Number |
26460292
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Research Collaborator |
BISWAS Kuntal
AKI Sho
Kuda Yuichi
MOHRI Hiromi
SHIMIZU Shouta
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | PI3キナーゼ / ホスフォイノシチド / 血管内皮細胞 / KOマウス / 血管炎症 / 細胞内小胞輸送 / ノックアウトマウス / 動脈硬化 / 解離性大動脈瘤 / イノシトールリン脂質 / 血管新生 / PI(3)Pホスファターゼ / 粥状動脈硬化 / 血管透過性 / PI(3)P ホスファターゼ |
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| Outline of Final Research Achievements |
Phosphatidylinositol 3-kinases (PI3Ks) are the family of lipid kinases responsible for the generation of 3’-phosphoinositides. We have recently reported that class II a-isoform PI3K (C2a) plays a crucial role in developmental and pathological angiogenesis, and is indispensable for the maintenance of the VE-cadherin-mediated EC barrier function and receptor endocytosis. These defects result in impaired vascular homeostasis under the pathophysiological conditions. In this study, we found that among myotubularin-related protein (MTMR) family members, the expression of MTMRx is relatively abundant in ECs. We found that, in HUVECs, MTMRx was localized mainly in the endosomes. Next we analyzed the phenotype of MTMRx global KO mice. MTMRx mice showed normally development but die perinatally. We conclude that C2a regulates vascular homeostasis through controlling PI(3)P+-membrane trafficking, cooperating with MTMRx in ECs.
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Report
(4 results)
Research Products
(18 results)
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[Presentation] Role of S1P2 in inflammation and fibrosis2015
Author(s)
Yoh Takuwa Juanjuan Zhao, Yasuo, Okamoto, Kazuaki Yoshioka and Noriko Takuwa
Organizer
14 th International Conference on Bioactive Lipids in Cancer, Inflammation, and Related Diseases
Place of Presentation
Sofitel Chain Bridge Hotel（ハンガリー ブタペスト）
Year and Date
2015-07-12
Related Report
Int'l Joint Research / Invited
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