Exploring the mechanisms for neuronal migration and intercellular communication steered by ion channels in the embryonic brain

• Project/Area Number: 26460293
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Akita Tenpei 浜松医科大学, 医学部, 准教授 (00522202)
• Co-Investigator(Renkei-kenkyūsha): FUKUDA Atsuo 浜松医科大学, 医学部, 教授 (50254272)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: イオンチャネル / 胎生期 / 大脳皮質形成 / GABA / 細胞移動 / 移動誘導因子 / 移動忌避因子 / 細胞内カルシウムシグナリング / 細胞内Ca2+シグナリング / 神経発達制御機構 / 細胞間情報伝達 / パッチクランプ / タウリン

Outline of Final Research Achievements

To elucidate the mechanisms for neuronal migration and intercellular communication steered by ion channels in the embryonic brain, we investigated the mechanisms of ion channel activation induced by the action of guidance molecules that promote migration or repulsion of GABAergic interneuron precursors during development of the cerebral cortex in embryos. We found that potassium ion channels are suppressed and anion channels are activated during migration, and that the regulation of ion channels during both migration and repulsion might be mediated by the rises in intracellular calcium ion concentration.

Research Products

• [Journal Article] Ion channels, guidance molecules, intracellular signaling and transcription factors regulating nervous and vascular system development. (2015)
  • Author(s): Akita, T., Kumada, T., Yoshihara, S. I., Egea, J., and Yamagishi, S.
  • Journal Title: J Physiol Sci. Volume: 66 Issue: 2 Pages: 175-188
  • DOI: 10.1007/s12576-015-0416-1
  • NAID: 40020800266
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Control of cortical neuronal migration by glutamate and GABA. (2015)
  • Author(s): Luhmann, H.J., Kilb, W. and Fukuda, A.
  • Journal Title: Frontiers in Cellular Neuroscience Volume: 9 Pages: 4-4
  • DOI: 10.3389/fncel.2015.00004
  • Peer Reviewed / Open Access
• [Journal Article] Characteristics and roles of the volume-sensitive outwardly rectifying (VSOR) anion channel in the central nervous system. (2014)
  • Author(s): T. Akita & Y. Okada
  • Journal Title: Neuroscience Volume: 275 Pages: 211-231
  • DOI: 10.1016/j.neuroscience.2014.06.015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 生理学者への歩み－カエルの神経から遺伝子疾患に至るまで－ (2016)
  • Author(s): 秋田 天平
  • Organizer: 第13回 日韓脳科学・心筋・平滑筋合同シンポジウム関連講演会
  • Place of Presentation: 国立大学法人佐賀大学医学部
  • Year and Date: 2016-08-24
  • Invited
• [Presentation] Roles of volume-regulated anion channels during neuronal migration in the developing brain. (2015)
  • Author(s): Akita T, Furukawa T & Fukuda A
  • Organizer: 第92回日本生理学会大会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-03-23
• [Presentation] 母体由来タウリンによるトニックGABAA受容体刺激が胎仔脳の放射状細胞移動に及ぼす影響 (2014)
  • Author(s): 福田 敦夫，古川 智範，山田 順子，秋田 天平，松島 芳隆，柳川 右千夫
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-12