Lipid regulation on receptor-operated ion channels and its tuning by Ca2+
| Project/Area Number |
26460296
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Kyoto University |
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Principal Investigator |
Masayuki Mori 京都大学, 工学研究科, 准教授 (80342640)
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| Co-Investigator(Renkei-kenkyūsha) |
Inoue Ryuji 福岡大学, 医学部, 教授 (30232573)
Mori Yasuo 京都大学, 工学研究科, 教授 (80212265)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | TRPチャネル / PIP2 / G蛋白質受容体 / 脂質 / カルシウムシグナル / 平滑筋 / Ca2+シグナル / Calmodulin / イノシトール代謝 / ＴＲＰチャネル / パッチクランプ / ＦＲＥＴ / ＰＩＰ２ / 非選択的カチオンチャネル / ＦＳＧＳ |
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| Outline of Final Research Achievements |
Phosphatidylinositol-4,5-bisphosphate (PIP2) has been recognized as a common regulator of TRP ion channels. However, the regulation diversification, the regulatory mechanism may not be identical because of complexity of its effective sites and functional outcome.
By extensive mutational study to measure kinetics from open to close as an index of PIP2 association, we newly identified Pre-S1 segment as PIP2 dependent gating along with N-terminal PH like domain Ankyrin domain, TRP box and C-terminal PIPs sensing region. In addition, we also found a conserved polarity change elements which possess Proline residues near TRP box. These findings could lead to further study to understand fundamental mechanism of PIP2 regulation on ion channels and its physiological imporatnces.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Screening of Transient Receptor Potential Canonical Channel Activators Identifies Novel Neurotrophic Piperazine Compounds.2016
Author(s)
Sawamura S, Hatano M, Takada Y, Hino K, Kawamura T, Tanikawa J, Nakagawa H, Hase H, Nakao A, Hirano M, Rotrattanadumrong R, Kiyonaka S, Mori MX, Nishida M, Hu Y, Inoue R, Nagata R, Mori Y.
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Journal Title
Mol Pharmacol.
Volume: 89
Pages: 348-363
Related Report
Peer Reviewed
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[Journal Article] TRPV4 channel activity is modulated by direct interaction of the ankyrin domain to PI(4,5)P22014
Author(s)
Nobuaki Takahashi, Sayaka Hamada-Nakahara, Yuzuru Itoh, Kazuhiro Takemura, Atsushi Shimada,Yoshifumi Ueda, Manabu Kitamata, Rei Matsuoka, Kyoko Hanawa-Suetsugu, Yosuke Senju, Masayuki X. Mori, Shigeki Kiyonaka, Daisuke Kohda, Akio Kitao, Yasuo Mori and Shiro Suetsugu
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Journal Title
Nature Communications
Volume: 5
Issue: 1
Pages: 4994-4994
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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