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Finding the cesium permeation pathway of cell membrane which contributes the accumulation of cesium into the organs.

Research Project

Project/Area Number 26460299
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General physiology
Research InstitutionFukushima Medical University

Principal Investigator

Hazama Akihiro  福島県立医科大学, 医学部, 教授 (60218394)

Co-Investigator(Kenkyū-buntansha) 三宅 将生  福島県立医科大学, 医学部, 助教 (00381385)
小林 大輔  福島県立医科大学, 医学部, 助教 (40528080)
勝田 新一郎  福島県立医科大学, 医学部, 准教授 (80285022)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsセシウム / HeLa細胞 / 細胞増殖 / HeLa / 細胞増殖抑制 / 輸送体 / トランスポータ / チャネル / Cs / イオンチャネル / ion channel / transporter
Outline of Final Research Achievements

We use HeLa cells as a model of Cs accumulation. We found that Cs could suppress HeLa cell proliferation in a dose-dependent manner (FEBS Lett. 2017 Mar;591(5):718-727). In this report Cs accumulation in HeLa cells was demonstrated and the enzymes for glycolysis including GAPDH (glyceraldehyde-3-phosphate dehydrogenase) , LDH(Lactate dehydrogenase), PK(Pyruvate kinase) were suppressed by Cs. We selected the candidates of Cs permeation pathway, including KCNH family, TRPC family and TRPM family. Expression levels of those candidates were examined by RT-PCR method in different cell lines. KCNH3, TRPC1, TRPC4, TRPM4, TRPM7 were considered as the Cs permeation pathway. We examined the change of expression level of those channels in HeLa cells by application of Cs. TRPC1 and TRPM7 were increased by Cs. Those channels are considered as the strong candidate for Cs permeation pathway.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Cesium reversibly suppresses HeLa cell proliferation by inhibiting cellular metabolism.2017

    • Author(s)
      Kobayashi D, Kakinouchi K, Nagae T, Nagai T, Shimura K, Hazama A.
    • Journal Title

      FEBS Lett.

      Volume: 591 Pages: 718-727

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] セシウムがHeLa細胞の代謝酵素発現に与える影響2017

    • Author(s)
      挾間章博、熊谷悠、久米佑佳、小林大輔
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      浜松
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Suppression of the HeLa cell proliferation by Cs without the effect on the membrane potential2016

    • Author(s)
      挾間章博
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
  • [Presentation] Csによる細胞増殖阻害時に何が起こっているか?2015

    • Author(s)
      小林大輔、垣野内景、長江智紀、挾間章博
    • Organizer
      日本生理学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] Cs+イオンの細胞増殖抑制効果における細胞種依存性の検討2015

    • Author(s)
      垣野内景、小林大輔、挾間章博
    • Organizer
      日本生理学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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