| Project/Area Number |
26460299
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General physiology
|
|---|
| Research Institution | Fukushima Medical University |
|---|
Principal Investigator |
Hazama Akihiro 福島県立医科大学, 医学部, 教授 (60218394)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
三宅 将生 福島県立医科大学, 医学部, 助教 (00381385)
小林 大輔 福島県立医科大学, 医学部, 助教 (40528080)
勝田 新一郎 福島県立医科大学, 医学部, 准教授 (80285022)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | セシウム / HeLa細胞 / 細胞増殖 / HeLa / 細胞増殖抑制 / 輸送体 / トランスポータ / チャネル / Cs / イオンチャネル / ion channel / transporter |
|---|
| Outline of Final Research Achievements |
We use HeLa cells as a model of Cs accumulation. We found that Cs could suppress HeLa cell proliferation in a dose-dependent manner (FEBS Lett. 2017 Mar;591(5):718-727). In this report Cs accumulation in HeLa cells was demonstrated and the enzymes for glycolysis including GAPDH (glyceraldehyde-3-phosphate dehydrogenase) , LDH(Lactate dehydrogenase), PK(Pyruvate kinase) were suppressed by Cs. We selected the candidates of Cs permeation pathway, including KCNH family, TRPC family and TRPM family. Expression levels of those candidates were examined by RT-PCR method in different cell lines. KCNH3, TRPC1, TRPC4, TRPM4, TRPM7 were considered as the Cs permeation pathway. We examined the change of expression level of those channels in HeLa cells by application of Cs. TRPC1 and TRPM7 were increased by Cs. Those channels are considered as the strong candidate for Cs permeation pathway.
|
