The physiological role of TASK channel in adrenaline secretion

• Project/Area Number: 26460306
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: Matsuoka Hidetada 産業医科大学, 医学部, 講師 (90374991)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: TASKチャネル / エンドサイトーシス / Src / TASK1 channel / endocytosis

Outline of Final Research Achievements

I investigated the function and physiological role of TASK1 channels in endocrine cells. Pharmacological and molecular biological analyses showed that TASK1 channels are regulated by the clathrin-dependent endocytosis in NGF- and muscarine-stimulated adrenal medullary and PC12 cells. Additionally, it showed that the molecular mechanisms regulating channel endocytosis differ between NGF-induced endocytosis and muscarine-induced endocytosis. The AM cells prepared from TASK1 KO mice indicated the decrease of pH sensitivity and adrenaline secretion, suggesting TASK1 channels play an important role in a sensor of acidosis and adrenaline secretion in AM cells.

Research Products

• [Journal Article] Functional interaction between BDNF and mGluR II in vitro: BDNF down-regulated mGluR II gene expression and an mGluR II agonist enhanced BDNF-induced BDNF gene expression in rat cerebral cortical neurons. (2017)
  • Author(s): Suzukia S, Koshimizu H, Adachi N, Matsuoka H, Fushimi S, Ono J, Ohta K, Mikia T.
  • Journal Title: Peptides Volume: 21 Pages: 42-49
  • DOI: 10.1016/j.peptides.2017.01.007
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] GABA signaling and neuroactive steroids in adrenal medullary chromaffin cells (2016)
  • Author(s): Harada K, Matsuoka H, Fujihara H, Ueta Y, Yanagawa Y, Inoue M
  • Journal Title: Frontiers in Cellular Neuroscience Volume: 18 Pages: 100-100
  • DOI: 10.3389/fncel.2016.00100
  • Peer Reviewed
• [Journal Article] Src mediates endocytosis of TWIK-related acid-sensitive K+ 1 channels in PC12 cells in response to nerve growth factor (2015)
  • Author(s): Matsuoka H and Inoue M
  • Journal Title: Am J Physiol Cell Physiol Volume: 309 Pages: 251-263
  • DOI: 10.1152/ajpcell.00354.2014
  • Peer Reviewed
• [Journal Article] Identification of muscarinic receptor subtypes involved in catecholamine secretion in adrenal medullary chromaffin cells by genetic deletion. (2015)
  • Author(s): Harada K, Matsuoka H, Miyata H, Matsui M, Inoue M
  • Journal Title: British Journal of Pharmacology Volume: 172 Pages: 1348-1359
  • DOI: 10.1111/bph.13011
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Fluorescent Visualization of the Hypothalamic Oxytocin Neurons Activated by CCK-8 in Rats Expressing c-fos-eGFP and Oxytocin-mRFP1 Fusion Transgenes (2014)
  • Author(s): Katoh, A. Shoguchi, K. Matsuoka, H. Yoshimura, M. Ohkubo, JI. Matsuura, T. Maruyama, T. Ishikura, T. Aritomi, T. Fujihara, H. Hashimoto, H. Suzuki, H. Murphy, D. & Ueta, Y.
  • Journal Title: Journal of Neuroendocrinology Volume: 26（5） Pages: 341-347
  • DOI: 10.1111/jne.12150
  • Peer Reviewed / Open Access
• [Presentation] Molecular mechanism for NGF-induced endocytosis of TASK1 channels in rat adrenal medullary cells and PC12 cells (2016)
  • Author(s): Matsuoka H and Inoue M
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター （北海道札幌市）
  • Year and Date: 2016-03-22
• [Presentation] 副腎髄質細胞およびPC12 細胞におけるTASK1チャネルエンドサイトーシスの分子機構 (2015)
  • Author(s): 松岡秀忠、原田景太、井上真澄
  • Organizer: 第120回日本解剖学会総会･全国学術集会・第92回日本生理学会大会 合同大会
  • Place of Presentation: 兵庫県神戸市 神戸国際会議場・展示場
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Src mediates endocytosis of TWIK-related acid-sensitive K+ channels in PC12 cells in response to nerve growth factor. (2014)
  • Author(s): Hidetada Matsuoka and Masumi Inoue
  • Organizer: Neuroscience 2014
  • Place of Presentation: Walter E. Washington Convention Center, USA
  • Year and Date: 2014-11-15 – 2014-11-19