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Analysis of the voltage dependence of Gq coupled receptors

Research Project

Project/Area Number 26460307
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General physiology
Research InstitutionNational Institute for Physiological Sciences

Principal Investigator

MICHIHIRO TATEYAMA  生理学研究所, 分子細胞生理研究領域, 准教授 (30276472)

Co-Investigator(Renkei-kenkyūsha) NAKAJO Koichi  大阪医科大学, 医学部, 准教授 (80390699)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
KeywordsGタンパク質共役型受容体 / FRET / 膜電位依存性 / 構造変化 / GPRC / 電位依存性
Outline of Final Research Achievements

It has been reported that the ligand affinities of several G protein coupled receptors are changed depending on the membrane potential. Here we investigated the effects of Gq coupled receptors on the KCNK13 channel current and found that in the purinergic P2Y1R concentration-response relationships were different when the holding potential was different. The voltage dependent difference was abolished when the aspartate residue at the transmembrane domain was mutated to alanine or asparagine. Similar results were obtained from the analysis of FRET between P2Y1R-YFP and CFP-Gq. FRET efficiency was increased by the application of agonist in a concentration dependent manner, and the concentration-FRET curve was leftward shifted by the depolarization of the membrane potential. The shift was not observed when the aspartate residue was mutated to aspargine.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Stabilizing effects of G protein on the active conformation of adenosine A1 receptor differ depending on G protein type2016

    • Author(s)
      Tateyama M, Kubo Y
    • Journal Title

      European Journal of Pharmacology

      Volume: 788 Pages: 122-131

    • DOI

      10.1016/j.ejphar.2016.06.025

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Auto-oxidation products of epigallo-catechin gallate activate TRPA1 and TRPV1 in sensory neurons.2015

    • Author(s)
      Kurogi, M., Kawai, Y., Nagatomo, K., Tateyama, M., Kubo, Y. and Saitoh, O.
    • Journal Title

      Chemical Senses

      Volume: 40(1) Issue: 1 Pages: 27-46

    • DOI

      10.1093/chemse/bju057

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] FRET analysis of the activation of GPCR2014

    • Author(s)
      立山充博、久保義弘
    • Journal Title

      Folia Pharmacologica Japonica

      Volume: 143 Issue: 5 Pages: 249-253

    • DOI

      10.1254/fpj.143.249

    • NAID

      130004827697

    • ISSN
      0015-5691, 1347-8397
    • Related Report
      2014 Research-status Report
  • [Presentation] FRET analyses of voltage dependence of P2Y1R2017

    • Author(s)
      立山充博、久保義弘
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      アクトシティ浜松(静岡県浜松市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Regulation of KCNK13 channel by Gi/o or Gq coupled receptors2016

    • Author(s)
      立山充博、久保義弘
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      北海道・札幌市・札幌コンベンションセンタ-
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Analyses of the voltage dependence and its structural determinant of the P2Y1 purinergic receptor.2016

    • Author(s)
      王 培冰、立山充博、久保義弘
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      北海道・札幌市・札幌コンベンションセンタ-
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] The Gi/o coupled muscarinic receptors form complex with the G protein gated inwardly rectifying potassium channel.2015

    • Author(s)
      Tateyama M、Kubo Y
    • Organizer
      8th FAOPS
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2015-11-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] The Gi/o coupled muscarinic receptors form complex with the G protein gated inwardly rectifying potassium channel for the efficient channel activation.2015

    • Author(s)
      立山充博、久保義弘
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      兵庫県・神戸市・神戸国際展示場
    • Year and Date
      2015-07-28
    • Related Report
      2015 Research-status Report
  • [Presentation] The Gi/o coupled muscarinic receptors form signaling complex with the G protein dependent inwardly rectifying potassium channe2015

    • Author(s)
      立山充博、久保義弘
    • Organizer
      第92回日本生理学会大会
    • Place of Presentation
      神戸国際展示場(兵庫県神戸市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] The KCNK13 channel current is increased by the activation of either the Gi/o- or the Gq-coupled receptor.2015

    • Author(s)
      松原美紀、立山充博、久保義弘
    • Organizer
      第92回日本生理学会大会
    • Place of Presentation
      神戸国際展示場(兵庫県神戸市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] Activation efficiency of the G protein gated inwardly rectifying potassium channel depends on distance from the Gq but not Gi/o coupled receptors2014

    • Author(s)
      立山充博、久保義弘
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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