| Project/Area Number |
26460308
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SUGIYA Hiroshi 日本大学, 生物資源科学部, 教授 (20050114)
HASHIMOTO Sadamitsu 東京歯科大学, 教養部, 教授 (10201708)
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| Research Collaborator |
NARITA Takanori 日本大学, 生物資源科学部, 講師 (70453884)
Steward Martin C. University of Manchester, School of Life Science, Reader
Castagnola Massimo Catholic University of Rome, Biochemistry, Professor
Ekström Jörgen University of Götebörg, Pharmacology, Professor
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 唾液腺 / タイト結合 / 傍細胞輸送 / 血管還流唾液腺 / 水輸送 / 細胞内信号 / 蛍光物質分泌 / 駆動力 / 血管灌流唾液腺 |
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| Outline of Final Research Achievements |
Paracellular transport is domminant during sustained stimulation of muscarinic receptor. Using various size of fluorescent marker, the relationship between fluid secretion and marker riatio in perfusate/saliva, whch revealed the paracellular route for various size of molecules is same. The driving force for paracellular fluid transport was assessed mainly hydrostatic pressure across tight junction. For measurement of vibration near the tight junction, the movement of gland by peristaltic pump was monitored three-demensionally in reference to the position of fluorescent bead fixed inside of the vasculature. In addition, the capillary was observed in the perfused gland morphologically. The number of capillary increased by muscarinic stimulation, suggesting that the increase in the area of vascular bed, causing a pressure decrease in the feeding artery but increase in the pressure surrounding acinar region due to th e increase in fluid flow across capillary endothelial cells.
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