| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We investigated the effect of AVP on reciprocal synaptic responses (DDI) measured from mitral cells in slice preparations from mice AOB with using the patch-clamp technique. We demonstrated that AVP significantly reduced the IPSCs triggered by applying a depolarizing voltage step from -70 mV in Mg2+-free solution.
To determine the contribution of different AVP receptor subtypes (V1a and V1b) to reduce the IPSCs, effects of an agonist and antagonists for V1Rs on the IPSCs were tested. An agonist for V1a receptors, [Phe2, Orn8]-vasotocin mimicked the AVP action on the IPSCs. The suppressive effect of AVP on the IPSCs was diminished by an antagonist for V1a receptors, Manning compound, while an antagonist for V1b receptors, SSR149415 unaffected the effects of it. The present results suggest that AVP modulates reciprocal transmission between mitral and granule cells through AVP V1a receptors.
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