| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
(1) We found that the plasmalogen (Pls)-induced activation of protein kinases was mediated by at least 5 orphan G-protein-coupled receptors (GPCRs), GPR1, GPR19, GPR21, GPR27 and GPR61 using knockdown and overexpression cells (PLoS ONE, 11: e0150846, 2016). (2) We found that inflammatory stimuli reduced Pls contents by downregulation of Pls synthesizing enzyme, GNPAT, through the NF-κB-mediated recruitment of c-Myc onto the GNPAT promoter in both murine and human cell lines. (J Neurosci, 37 (15): 4074-4092, 2017). (3) Knock down of GNPAT in the mouse hippocampus impaired spatial memory and decreased brain-derived neurotrophic factor (BDNF) expression. Pls-containing diet in mice increased hippocampal Pls, enhanced spatial memory with high expressions of phosphorylated (p-) Akt, p-CREB and BDNF along with enhanced hippocampal neurogenesis, spine formation, and long term potentiation. (in submission).
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